| 光谱学与光谱分析 |
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| Studies on Application and Mechanism of Energy Transfer System of Acridine Orange(AO)-Rhodamine B(RB) Dimer in the Determination of DNA |
| GAO Feng, ZHU Chang-qing, WANG Lun |
| College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, China |
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Abstract Not only dye monomer is the form of energy transfer but also dye dimer is the effective form of energy transfer. In this work, the energy transfer system of acridine orange(AO)-rhodamine B(RB) dimer was built for the determination of DNA as fluorescence probe. At the same time, the mechanism was also considered. Under the optimum experimental conditions, the linear range of this assay was 0.33-1.33 mg·L-1 for fish sperm DNA and 0.33-3.33 mg·L-1 for calf thymus DNA, the detection limit was 1.63×10-3 mg·L-1 for fish sperm DNA and 1.52×10-3 mg·L-1 for calf thymus DNA, respectively. The RSD for the determination of 1.00 mg·L-1 DNA was 2.40% for fsDNA and 2.00% for ctDNA, respectively. The method was used for the determination of DNA in synthetic samples with satisfied results.
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Received: 2002-09-16
Accepted: 2003-01-06
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Corresponding Authors:
WANG Lun
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| Cite this article: |
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GAO Feng,ZHU Chang-qing,WANG Lun. Studies on Application and Mechanism of Energy Transfer System of Acridine Orange(AO)-Rhodamine B(RB) Dimer in the Determination of DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 85-88.
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http://www.gpxygpfx.com/EN/Y2004/V24/I01/85 |
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