光谱学与光谱分析 |
|
|
|
|
|
Studies on the Interaction of Metalic Complex and DNA by the Spectral Methods |
LIN Qiu-yue,HU Rui-ding,ZHENG Xiao-hua |
Department of Chemistry,College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China |
|
|
Abstract The interaction of [Cu(A)2]2+ (A=phen,bpy,en) complex ion and ct-DNA was investigated by using absorption spectra and fluorescence spectra. Based on the measurement of A260 in the different pH mediums, the alkaline denaturation curves of DNA in the absence and in the presence of [Cu(phen)2]2+ complex ion were obtained. The result showed that the alkaline denaturation for [Cu(phen)2]2+ complex ion occured at a higher pH than that for DNA alone. The absorption of EB-DNA system in the presence of [Cu(phen)2]2+ showed that [Cu(phen)2]2+ could partly sustain the place of EB. The result of fluorescence spectra suggested that the [Cu(phen)2]2+ complex ion could make the fluorescence intensity of EB-DNA system to decreased sharply. The effect of [Fe(CN)6]4- on the emission of [Cu(phen)2]2+and [Cu(phen)2]2+-DNA system was studied. All the experimental results indicated that the intercalative binding mode was the major mode for the interaction of [Cu(phen)2]2+ complex ion and DNA,and the size of ligands can effect the binding of complexes to DNA.
|
Received: 2003-12-02
Accepted: 2004-03-28
|
|
Corresponding Authors:
LIN Qiu-yue
|
|
Cite this article: |
LIN Qiu-yue,HU Rui-ding,ZHENG Xiao-hua. Studies on the Interaction of Metalic Complex and DNA by the Spectral Methods [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(08): 988-990.
|
|
|
|
URL: |
http://www.gpxygpfx.com/EN/Y2004/V24/I08/988 |
[1] Barton J K, Goldherg J M, Kumar C V et al. J. Am. Chem. Soc., 1986, 108(8): 2081. [2] SONG Yu-min,KANG Jing-wan,GAO Jin-zhang(宋玉民, 康敬万,高锦章). Chinese Journal of Inorganic Chemistry(无机化学学报),2000,16(1): 53. [3] 日本化学会(著). Synthetical Handbook for Inorganic Compounds(无机化合物合成手册(第三版)). CAO Hui-min(曹惠民(译)). Beijing:Chemical Industry Press(北京:化学工业出版社),1988. 379. [4] YE Yong,HU Ji-ming,ZENG Yun-e(叶 勇,胡继明,曾云鹗). Chinese Journal of Analytical Chemistry(分析化学),2000,28(7): 798. [5] LI Wen-you,ZHU Shou-tian,HE Xi-wen et al(李文友,朱守田,何锡文等). Acta Chimica Sinica(化学学报), 2002,60(1): 105. [6] Pal M K, Ghosh J K. Spectrochim. Acta, Part A,1995, 51: 489.
|
[1] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, YUE Yuan-bo2, HU Xue-qiang2, CHEN Yu2, LI Xiao-jia1, 2*. The Detection of Mercury in Solutions After Thermal Desorption-
Enrichment by Energy Dispersive X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1117-1121. |
[2] |
WANG Jun1, WANG Zhou-li2, CHENG Jing-jing1. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 904-909. |
[3] |
XU Yang-biao, WANG Hai-shui*. The Removal of Water Peaks From IR Spectra of BSA Aqueous Solution by Two ATR Background Samples and Thermal Behavior of Proteins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 642-646. |
[4] |
LÜ Jia-nan, LI Jun-sheng*, HUANG Guo-xia, YAN Liu-juan, MA Ji. Spectroscopic Analysis on the Interaction of Chrysene With Herring Sperm DNA and Its Influence Factors[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 210-214. |
[5] |
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827. |
[6] |
ZHANG Chen-ling, JIA Na*, LIU Jia, LIU Bing-bing, HAN Mei. Investigation of Lithium Analysis in Geothermal Water by Inductively Coupled Plasma Optical Emission Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3876-3880. |
[7] |
LIU Jia-cheng1, 2, HU Bing-liang1, YU Tao1*, WANG Xue-ji1, DU Jian1, LIU Hong1, LIU Xiao1, HUANG Qi-xing3. Nonlinear Full-Spectrum Quantitative Analysis Algorithm of Complex Water Based on IERT[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3922-3930. |
[8] |
LI Zheng-kai1, CHEN Lei1*, WANG Mei-qi1, SONG Peng2, 3, YANG Kun1, ZENG Wen1. Diagnosis of Atmospheric Pressure Argon/Air Needle-Ring Dielectric Barrier Discharge Emission Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3307-3310. |
[9] |
LIU Yu1, LI Zeng-wei2, DENG Zhi-peng1, ZHANG Qing-xian1*, ZOU Li-kou2*. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2817-2822. |
[10] |
WANG Xiao-xia1,3*, WU Hao1, NIE Zhi-hua2, MA Li-tong1,3*, CUI Jin-long1, SAI Hua-zheng1, CHENG Jian-guo1. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2904-2910. |
[11] |
JIA Gui-hong1, 2*, ZHANG Jian-jun1, ZHENG Hai-ming2. Research on FFT+FT Spectrum Zooming Method for Differential Optical Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(07): 2116-2121. |
[12] |
ZHANG Chuan-ying1, PENG Xin1*, RAO Heng-jun2, QI Wei2, SU Rong-xin2, HE Zhi-min2. Spectroscopic Studies on the Interaction Between Salvianolic Acid B and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1701-1707. |
[13] |
SONG Peng1,3, LI Zheng-kai2, CHEN Lei2*, WANG Xiao-fang1, LONG Wu-qiang1, ZENG Wen2. Diagnosis of Atmospheric Pressure Helium Cryogenic Plasma Jet[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1874-1879. |
[14] |
ZHANG Peng-peng1, 2, HU Meng-ying1, 2, XU Jin-li1, 2*, CHEN Wei-ming1, 2, GU Xue1, 2, ZHANG Ling-huo1, 2, BAI Jin-feng1, 2, ZHANG Qin1, 2. Determination of Available Boron in Soil by ICP-OES With Boiling Water Extraction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1925-1929. |
[15] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
|
|
|
|