光谱学与光谱分析 |
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Reaction Mechanism of Cefotaxime with Human Serum Albumin |
LIU Luo-sheng1, WANG Xing-po1, ZHAO Quan-qin1, ZHANG Yu-yi2 |
1. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China 2. School of Medicine, Shandong University, Jinan 250012, China |
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Abstract The reaction mechanism of cefotaxime with human serum albumin (HSA) and the affinity between cefotaxime and β-lactamase were investigated by spectrometry and spectrofluorimetry. The interaction dissociation constants of human serum albumin and cefotaxime were determined from a double reciprocal Lineweaver-Burk plot. The binding distance and transfer efficiency between cefotaxime and HSA were also obtained according to the theory of Frster non-radiation energy transfer. The result suggested that the main binding force between cefotaxime and HSA is electrostatic force interaction. The high β-lactamase stability of cefotaxime may be correlative with its molecular structure. The antibiotic activity and valence are connected with transfer efficiency and dissociation constant. The effect of cefotaxime on the conformation of HSA was also analyzed using synchronous fluorescence spectrometry.
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Received: 2005-03-16
Accepted: 2005-10-06
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Corresponding Authors:
LIU Luo-sheng
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Cite this article: |
LIU Luo-sheng,WANG Xing-po,ZHAO Quan-qin, et al. Reaction Mechanism of Cefotaxime with Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(06): 1130-1133.
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URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I06/1130 |
[1] Gelamo E L, Tabak M. Spectrochimica Acta, A,2000, 56: 2255. [2] Sulkowska A, Bojko B. J. Rownicka et.al. Journal of Molecular Structure, 2003, 651-653: 237. [3] AnnaSulkowska Journal of Molecular Structure, 2002, 614: 227. [4] YANG Min,LI Jia-tai(杨 敏,李家泰). Chinese J. of Antibotics(中国抗生素杂志), 1999, 24(5):353. [5] DU Xiu-lian, LI Rong-chang, WANG Kui(杜秀莲,李荣昌,王 夔). Chinese Science Bulletin(科学通报), 2001, 46(5):394. [6] LIU Luo-sheng,ZHAO Li,GE Wei-ying(刘洛生, 赵 丽, 葛蔚颖). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4):769. [7] LIU Luo-sheng,ZHANG Yu-yi,WANG Xing-po(刘洛生, 张虞毅, 王兴坡). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(9):1490. [8] LIU Luo-sheng,GE Wei-ying,MENG Qing-mei(刘洛生, 葛蔚颖, 孟庆梅). Acta Academia Medicine Shandong(山东医科大学学报), 1997, 35(4):257.
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