| 光谱学与光谱分析 |
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| Study on the pH-Sensitive Secondary Structure of γ-PGA Embedded with Magnetite Nanoparticles |
| ZHANG Ju-hua1, 2, XING Jian-min1*, JIANG Yang-yang1, 2, GAO Hong-shuai1, 2, LIU Hui-zhou1 |
1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract The conformational transition of poly γ-glutamic acid (γ-PGA) embedded with magnetite nanoparticles under various pH conditions was investigated by Fourier transform infrared spectroscopy (FTIR). The secondary structure content was determined through the analysis of amide Ⅰ bands of Fourier deconvolution spectra, secondary derivative spectra and the Gaussian curve fitting of the original infrared spectra. The results showed that the conformation of the γ-PGA was affected by solution pH. The total contents of β-sheet and β-turn were higher than 65％, while α-helix and random coil were low. The content of β-turn increased with increasing pH, while the β-sheet decreased. Additionally, the zeta potential results showed that the pH-sensitive secondary structure of γ-PGA had influence on the stability of suspension of magnetic γ-PGA nanospheres. The minimum value of zeta potential (-35.4 mV) was obtained at pH 10.2.
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Received: 2008-05-10
Accepted: 2008-08-20
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Corresponding Authors:
XING Jian-min
E-mail: hzliu@home.ipe.ac.cn
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