| 光谱学与光谱分析 |
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| ICCD-Based Fast Fluorescence Micro-Imaging Technique and Preliminary Application in Living Cells |
| LIN Dan-ying, SUN Yun-xu, MA Wan-yun*, HAN Dong |
| The Key Laboratory of Atomic and Molecular Nanosciences, Ministry of Education, Tsinghua University, Beijing 100084, China |
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Abstract A fast fluorescence micro-imaging system using mainly intensified charge couple device(ICCD), argon-ion laser, and xenon lamp was set up, and its preliminary application in living cells was presented. Real-time observation and imaging of fast concentration and distribution changing of intracellular Ca2+ labeled by Fluo-3, a fluorescent Ca2+ indicator, in the proliferation process of rat cerebral micro-vessels endothelial cells(rCMECs) were carried out, and curves of artificial intensity versus imaging sequence of four typical points were obtained. It is shown that the ICCD-based fast fluorescence micro-imaging system is a powerful tool for recording the real-time fast processes in living cells.
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Received: 2004-12-08
Accepted: 2005-03-20
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Corresponding Authors:
MA Wan-yun
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| Cite this article: |
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LIN Dan-ying,SUN Yun-xu,MA Wan-yun, et al. ICCD-Based Fast Fluorescence Micro-Imaging Technique and Preliminary Application in Living Cells [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 917-921.
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https://www.gpxygpfx.com/EN/Y2006/V26/I05/917 |
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