| 光谱学与光谱分析 |
|
|
|
|
|
| The Study of Cervical Cancer Cells Model Based on UV Absorption Spectrum |
| LIN Xiao-gang, PAN Ying-jun, GUO Yong-cai, ZHANG Wei, ZHAO Yi-shi |
| Opto-Electronic Technology and Systems Key Laboratory under the Ministry of Education, Chongqing University, Chongqing 400030, China |
|
|
|
|
Abstract Cell cycle is the most important process in life, and embodies all the physiological processes in the cell. In order to reflect the process of cell growth simply and accurately, the authors took cell cycle into account when the Hela cell spectral model was designed. The artificially induced cell synchronization method was employed to make Hela cell in G1, S, G2 and M phases of the cell cycle. The UV absorption spectra of these Hela cell samples were measured. The absorbability of aromatic amino acid, protein and nucleic acid in different stages of the cell cycle showed the changes in cell cycle. The subsection linear regression model of cell UV absorption spectrum was designed according to the relationship between the stage and the absorbance of samples in different phase at 204 and 260 nm. The models can be used to estimate the cell cycle after experimental verification and provide a new method for analyzing cell cycle and building cell model.
|
|
Received: 2008-03-29
Accepted: 2008-07-02
|
|
|
|
Corresponding Authors:
LIN Xiao-gang
E-mail: xglin@cqu.edu.cn
|
|
[1] SONG Jin-dan(宋今丹). Medical Cellular Biology(医学细胞生物学). Beijing: People’s Medical Publishing House(北京：人民卫生出版社), 2004. 259.
[2] Collier J R, Mcinerney D, Schnell S, et al. Journal of Theoretical Biology，2000，207：305.
[3] GUO Yao-jun(郭尧君). Technique of Spectral Photometer and Its Application in Biochemistry(分光光度技术及其在生物化学中的应用). Beijing：Science Press(北京：科学出版社)，1987. 224.
[4] Boydston-White S, Gopen T, Houser S, et al. Biospectroscopy，1999，5：219.
[5] Susie Boydston-White, MelissaRomeo, Tatyana Chernenko, et al. Biochimica et Biophysica Acta，2006，1758：908.
[6] Pagano M. Cell Cycle—Materials and Methods. Berlin Heidellberg: Springer-Verleg, 1996.
[7] Lew D J, Dulic V, Reed S I. Cell，1991，66：1197.
[8] Gui Jianfang, William S Lane, Fu Xiangdong. Nature，1994，369：678.
[9] GUI Jian-fang(桂建芳). The RNA Machining and Cell Cycle Contral(RNA加工与细胞周期调控). Beijing：Science Press(北京：科学出版社)，1998. 88.
[10] Azzedine Hammiche, Matthew J German, Rebecca Hewitt, et al. Biophysical Journal，2005，88：3699.
[11] Brian Mohlenhoff, Melissa Romeo, Max Diem, et al. Biophys J., 2005, 88(5): 3635.
[12] Azzedine Hammiche, Matthew J German, Rebecca Hewitt, et al. Biophys J. 2005, 88(5): 3699.
[13] Mourant J R, Yamada Y R, Carpenter S, et al. Biophys. J., 2003, 85(3): 1938.
[14] Tokuko Haraguchi, Toru Kaneda, Yasushi Hiraoka. Genes to Cells, 1997, 2: 369. |
| [1] |
LIU Dong1, SUN Xue-jun1*, ZHANG Chao1, HE Sai1, DU Jun-kai1, HUO Xiong-wei1, ZHENG Jian-bao1, ZHANG Shi-yun1, ZHANG Yuan-fu2, XU Yi-zhuang2*, WU Jin-guang2. Label-Free Monitoring 5-FU Induced SW 620 Cells Apoptosis Using FTIR Microspectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(12): 3169-3174. |
|
|
|
|
