|
|
|
|
|
|
| Visual Colorimetric Detection of Hg(Ⅱ) with Graphene Oxide Peroxidase-Like Activity |
| LIN Xi1, LU Yi-song2, YANG Sheng-yuan1*, LIU Lu-qun1, LI Fei-fei1, HE Shun-zhen1 |
1. College of Public Health，University of South China，Hengyang 421001，China
2. Affiliated Nanhua Hospital, University of South China, Hengyang 421001, China |
|
|
|
|
Abstract A new method for visual detection of trace Hg2+ ions was successfully established based on the single-stranded DNA (ssDNA) and T-Hg2+-T complex have different interaction with graphene oxide(GO), which owns mimetic enzyme catalytic performance. In NaAc-HAc buffer of pH 4.0, ssDNA can be adsorbed on the GO surface, which will efficiently decrease the peroxidase-like activity of GO to catalyze the oxidation of peroxidase substrate TMB to form blue product in the presence of H2O2, which presented a characteristic absorbance at 652 nm. When Hg2+ ions exist, the ssDNA formed double-stranded DNA via thymine-Hg2+-thymine complex coordination structure, which hardly absorbed on the surface of GO for the interactionbetween dsDNA and GO is much weaker, the peroxidase mimetic activity of GO is not affected, accompanying with the color darkening and increase of absorbance at 652 nm. Under the optimal conditions, the absorbance versus Hg2+ concentration is linear in the range from 3.26×10-8 to 9.0×10-7 mol·L-1, which can make a calibration graph. The regression equation is ΔA=41.75c(nmol·L-1)+0.048 7, r=0.997 3. The limit of detection is 9.79×10-9 mol·L-1. This method has the advantages of simplicity, cost saving and good specificity, thus can be applied for determinating mercury ion in the environment samples.
|
|
Received: 2017-10-09
Accepted: 2018-02-08
|
|
|
|
Corresponding Authors:
YANG Sheng-yuan
E-mail: yangshyhy@126.om
|
|
[1] Gonzalez-Raymat H, Liu G, Liriano C, et al. Environmental Pollution, 2017, 229: 69.
[2] Sundseth K, Pacyna J, Pacyna E, et al. International Journal of Environmental Research & Public Health, 2017, 14(1): 105.
[3] Bansod B, Kumar T, Thakur R, et al. Biosensors & Bioelectronics, 2017, 94: 443.
[4] Sun Z L, Du J J, Jing C Y. Journal of Environmental Sciences, 2016, 39: 134, doi: 10.1016/j.jes.2015.11.009.
[5] Pokhrel L R, Ettore N, Jacobs Z L, et al. Science of the Total Environment, 2017, 574: 1379.
[6] Simsek A E, Yayayuruk A E, Shahwan T, et al. Journal of Analytical Chemistry, 2017, 72(5): 551.
[7] Yuan X, Yang G, Ding Y, et al. Spectrochimica Acta Part B Atomic Spectroscopy, 2014, 93(2): 1.
[8] Liu Y M, Zhang F P, Jiao B Y, et al. Journal of Chromatography A, 2017, 1493: 1.
[9] Yao C H, Jiang S J, Sahayam A C, et al. Microchemical Journal, 2017, 133: 556.
[10] Zhang G, Wu L, Li C, et al. Review of Scientific Instruments, 2017, 88(7): 1045.
[11] SU Ce, CHANG Wen-wu, WU Jie(苏 策，常文武，吴 杰). Journal of Lanzhou University of Technology(兰州理工大学学报), 2015, 41(3): 78.
[12] Liang Y, Su J, Huang Y, et al. Analytical Sciences, 2016, 32(5): 565.
[13] Lu C H, Yang H H, Zhu C L, et al. Angew. Chem. Int. Edit.，2009, 48(26): 4785.
[14] Wang Y, Shang X, Liu J, et al. Talanta, 2017, 176: 652.
[15] Hu Y, Zhang Q, Guo Z, et al. Biosensors & Bioelectronics, 2017, 98: 91. |
| [1] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
| [2] |
ZHU Shao-hao1, SUN Xue-ping1, TAN Jing-ying1, YANG Dong-xu1, WANG Hai-xia2*, WANG Xiu-zhong1*. Study on a New Sensing Method of Colorimetric and Fluorescence Dual Modes for Pesticide Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2785-2791. |
| [3] |
ZHANG Liang1, ZHANG Ran2, CUI Li-li3, LI Tao1, GU Da-yong4, HE Jian-an2*, ZHANG Si-xiang1*. Rapid Modification of Surface Plasmon Resonance Sensor Chip by
Graphene Oxide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 795-800. |
| [4] |
MA Ji1, 2*, HUANG Guo-xia1, 2, LI Jun-sheng1, 2*, YAN Liu-juan1, 2, ZHANG Qian1. A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2795-2799. |
| [5] |
JULDEZ Nurlan1，2，3, SHEN Jian1，2，3, LENG Xiao-ting3, CHAI Yi-di1，2，3, WANG Shi-feng3, HU Yuan3, CUI Hao-yue3, WU Jing1，2，3*. Study on Measurement of Mercury Ion in Water by Thiamine-Fluorescence Excitation-Emission Matrix[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1846-1851. |
| [6] |
OU Li-juan, AN Xue-zhong, LUO Jian-xin, WANG Ling-yun, BO Heng, SUN Ai-ming, CHEN Lan-lan. High-Sensitive and Rapid Fluorescencet Detection of Hg2+ Based on Poly(adenine)-Templated Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 164-167. |
| [7] |
JI Bang1,2, ZHAO Wen-feng3, DUAN Jie-li4, FU Lan-hui1, MA Li-zhe3, YANG Zhou1*. Spectral Characteristics of Ag3PO4/GO on Nickel Foam and Photocatalytic Degradation of Ethylene Under Visible Light[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2743-2750. |
| [8] |
ZHANG Jia-qin1, FANG Zhi-hao2, LIANG Hui-e1*. Preparation and Spectral Analysis of Modern Anti-Ultraviolet Cotton Fabrics[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2770-2774. |
| [9] |
WEI Gang1, GU Zheng-ye1, GONG Shui-shui1, GUANG Shan-yi2, KE Fu-you1, XU Hong-yao1*. Determination of the Oxidizability on the Surface of the Graphene Oxide Layer by Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1722-1727. |
| [10] |
LI Zhi-yuan1, LIU Xue-lian1,2, ZHENG An-dong1, WANG Guo-dong1, XIA Guo1,3, LU Hong-bo1,2*. Research on the Spectral Analysis and Stability of Ultraviolet-Enhanced Thin Films with Stable and Tunable Graphene Oxide-Rare Earth Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(02): 379-384. |
| [11] |
YANG Lei, LIN Bin-bin, ZHENG Qi-wei, WU Shu-lan, ZHENG Bing-yun, ZHU Zhi-fei, HU Wen-ying. Its Photochemical Recognition to Hg2+ and Preparation of Nitrogen and Sulfur-Codoped Carbon Dots by Sulfanilamide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(11): 3388-3394. |
| [12] |
YANCHEN Zhou-yan, HONG Cheng-yi, LIN Zheng-zhong, HUANG Zhi-yong*. Detection of Hg(Ⅱ) in Water with Ratio Fluorescent Paper Strip Based on Carbon Dots and RhB[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(11): 3426-3432. |
| [13] |
ZHU Xi-yu1, 2, WANG Ruo-yu2, ZHOU Xiao-hong2*, TAN Ai-juan1*, WEN Xiao-gang3*. Functional Nucleic Acid Based Fluorescent Biosensing Method for Hg2+ Detection in Water Samples[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3447-3451. |
| [14] |
ZHAN Yan1, ZU Hong-ru1, HUANG Di1*, HU Chao-fan1,2*. Rapid Synthesis of Graphene Oxide Quantum Dots via Hydrothermal Strategy for Cell Imaging Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1458-1462. |
| [15] |
ZHANG Cai-hong, ZHOU Guan-ming*, ZHANG Lu-tao, LUO Dan, YU Lu, GAO Yi. An Application to Quantitative Analysis of Hg(Ⅱ) with L-Cysteine Molecular Probe by Surface-Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 117-122. |
|
|
|
|
