|
|
|
|
|
|
| Experimental Teaching Reform of Soil Analysis Under the Background of New Agricultural Science: Comparative Study of ATR-FTIR and FTIR
Technique |
| LÜ Feng-lian1, 2, SHAN Xiao-ling1, 2, LI Li-min1, ZHAO Ran1, 2, ZHENG Wei1* |
1. Key Laboratory of Plant Nutrition and the Agro-Environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China
2. Experiment Center of Resources and Environmental Science Research, College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China
|
|
|
|
|
Abstract Against the background of new agricultural construction, improving the practical innovation ability of undergraduates in agricultural colleges and universities has become the core goal of experimental curriculum reform. The traditional experimental course content is obsolete, and the technical method is single, which makes it difficult to meet the needs of new agricultural science for compound talents. In this study, Fourier transform infrared spectroscopy (FTIR) was used as a starting point to systematically compare the operational procedures, data characteristics, and teaching applicability of attenuated total reflection (ATR) and transmission (FTIR) for the first time in the determination of soil functional groups under different fertilization treatments. Based on the soil analysis experiment, a three-stage teaching mode of “basic cognition-method comparison-comprehensive application” was developed, with teaching conducted through group experiments and comparative analysis. The results showed that: ① The average peak height of the —OH functional group (3 400 cm-1) detected by the FTIR method was 0.32±0.04 (n=3), and the ratio of the C—H vibration peak area at 1 440 cm-1 was 3~4 times that of the ATR method, which was more suitable for accurate quantitative analysis. ② After the reform, the score rate of students in the final “ATR/FTIR method selection application question” increased from 62% to 89%, the success rate of FTIR tableting increased from 65% to 88%, and the error points of the self-recognition experiment increased from 1.8 to 4.2. ③ Under different fertilization treatments, SNPK treatment significantly reduced the content of soil aromatic compounds (ATR method: 3.16%±0.14% vs. CK: 4.27%±0.01%), and 50% NPK+50% M treatment significantly increased the content of phenols and alcohols (FTIR method: 15.35%±1.93% vs. CK: 13.12%±1.29%). Through the logical closed loop of “technical comparison-practical verification-innovative application”, this teaching mode significantly improves students' scientific research cognitive depth, operational innovation ability and scientific research thinking, more effectively connects the principle of FTIR technology with the needs of new agricultural soil research, optimizes the structure of agricultural resources and environment experiment course, and provides a replicable practical path for the cultivation of new agricultural cross-disciplinary talents.
|
|
Received: 2025-06-03
Accepted: 2025-09-19
|
|
|
|
Corresponding Authors:
ZHENG Wei
E-mail: zheng_wei@nwafu.edu.cn
|
|
[1] SHI Ding-fang，WU Neng-biao(石定芳，吴能表). China Agricultural Education(中国农业教育)，2021，22(1)：44.
[2] GUAN Shu-yan，MA Yi-yong，LIU Si-yan(关淑艳，马义勇，刘思言). Journal of Changchun Normal University(长春师范大学学报)，2023，42(12)：157.
[3] GUO Na，CAO Ya-bing，CAO Xi-bing，et al(郭 娜，曹亚兵，曹喜兵，等). Journal of Higher Education(高教学刊)，2024，10(36)：177.
[4] CHEN Yu-chun，WANG Guo-dong(陈遇春，王国栋). China Higher Education Research(中国高教研究)，2011，(6)：62.
[5] WU Shun，GUAN Jie(吴 顺，关 洁). Chinese Journal of Biotechnology(生物工程学报)，2023，39(11)：4742.
[6] ZHANG Ji-yu，XIE Wen-gang，ZHANG Jian-quan，et al(张吉宇，谢文刚，张建全，等). Pratacultural Science(草业科学)，2024，41(7)：1767.
[7] ZHENG Xiao-jian，GONG Cheng-liang，SHEN Wei-de(郑小坚，贡成良，沈卫德). Research and Exploration in Laboratory(实验室研究与探索)，2007，26(9)：84.
[8] ZHAO Jun-yu，QIN Zuo-yu，KE Qin，et al(赵隽宇，覃祚玉，柯 琴，等). Research of Soil and Water Conservation(水土保持研究)，2025，32(4)：68.
[9] DU Chang-wen，ZHOU Jian-min(杜昌文，周健民). Chinese Journal of Analytical Chemistry(分析化学)，2007，35(1)：119.
[10] QIAN Hong-yu，CHEN Zi-yan，FENG Xiao-xuan，et al(钱虹宇，陈子彦，冯小轩，等). Acta Agrestia Sinica(草地学报)，2024，32(12)：3715.
[11] CHEN Yu-bing，YU Shi-kang，LI Fu-cheng，et al(陈玉冰，俞世康，李富程，等). Jiangsu Agricultural Sciences(江苏农业科学)，2024，52(21)：260.
[12] ZHANG Ming-yang，ZHU Zhao-long，LI Hao-hao，et al(张明洋，朱兆龙，李好好，等). Research of Soil and Water Conservation(水土保持研究)，2022，29(6)：121.
[13] LI Xue-lan，LI De-cheng，ZHENG Guang-hui，et al(李学兰，李德成，郑光辉，等). Acta Pedologica Sinica(土壤学报)，2024，61(3)：687.
[14] PEI Zhi-fu，SHEN Qin-guo，FAN Ruo-yu，et al(裴志福，沈钦国，范若渝，等). Chinese Journal of Soil Science(土壤通报)，2024，55(5)：1264.
[15] WANG Yu，ZHANG Yi-ping，CHEN Si-gen(王 玉，张一平，陈思根). Journal of Northwest A&F University(Natural Science Edition)[西北农林科技大学学报（自然科学版）]，2003，31(1)：57.
[16] LÜ Feng-lian，SHAN Xiao-ling，ZHAO Ran，et al(吕凤莲，单晓玲，赵 冉，等). Chinese Journal of Soil Science(土壤通报)，2025，56(2)：381.
[17] ZOU Hao-lin，LUO Zong-chao，LI Bang-lin(邹浩琳，罗宗超，李帮林). Journal of Southwest China Normal University(Natural Science Edition)[西南师范大学学报（自然科学版）]，2021，46(9)：154.
[18] LI Zi-zhi(李子帙). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2012，29(3)：1649.
|
| [1] |
YANG Shan, CAI Xiu-qin, LIU Yu-han, WANG Wei. A Simple Measuring Method for Infrared Spectroscopy of Liquid Matters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2143-2147. |
| [2] |
YANG Shan, CAI Xiu-qin, ZHANG Yi-feng. A Comparative Study on the ATR and TR Methods of Infrared Spectroscopy of Solid Matters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2775-2780. |
| [3] |
LUO Ze-min, SHEN Andy, YANG Ming-xing . Study on Color Quantitative Expression, Replication and Color Origin of Gray-Purple Nephrite from Qinghai, China Based on Spectroscopy Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(03): 822-828. |
| [4] |
XING Jian1, 2, SUN Xiao-gang2, ZHOU Chen3, YUAN Gui-bin1, ZHANG Zhi-lin1 . Complex Refractive Index Measurement of Fly Ash Particles Using Suspension Spectral Transmission Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(12): 3371-3374. |
| [5] |
SUN Xiao-gang,TANG Hong,YUAN Gui-bin. Research on the Measurement of Flue-Dust Concentration in Vis, IR Spectral Region[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(10): 2268-2272. |
| [6] |
REN Yu1,2, XU Yi-zhuang1, ZHAO Ying3, YANG Li-min1, LI Qing-bo1, ZHANG Yuan-fu1, WENG Shi-fu1, SHI Jing-sen2, XU Duan-fu3, WU Jin-guang1*. A Comparative Study of Malignant Tissue Diagnosis Using ATR and Microscopy FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(08): 930-932. |
|
|
|
|
