1. Jiangxi Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology, Nanchang Institute of Technology, Nanchang 330099, China
2. School of Life Science, Anhui Agricultural University, Hefei 230036, China
Abstract：An ultrasonic extraction-multiskan spectrum-microtitration assay method has been developed to detect the total petroleum hydrocarbons in environmental matrix. With a study into the parameters and variables that affected the extraction and detection of petroleum, it was found that the optimal conditions for extraction and detection are as follows: the detecting wavelength being 304 nm, using petroleum ether as extracting reagent, soil and liquid ratio being 1∶4, ultrasonic extracting two times with each one lasting 20 minutes, and ultrasonic extracting power, 100 W. Under these conditions, the recovery rates of total petroleum hydrocarbons ranged from 88.4% to 101.6%, with all the relative standard deviations less than 4.7%. Compared to the traditional extracting method which involves the use of lots of organic reagents such as dichloromethane, it would be more environment-friendly, for it is less harmful to the environment. Replacing ultraviolet spectrophotometer with multiskan spectrum, the samples can be detected more easily and rapidly with a relatively small amount of solution. Consequently, this method proves to be an efficient, green, rapid and inexpensive approach for extraction and determination of total petroleum hydrocarbons from environmental samples, which can be widely used in the quantitative determination of the trace environmental samples of petroleum contamination.
Key words：Ultrasonic extraction; Multiskan spectrum; Microtitration assay; Total petroleum hydrocarbons
基金资助: the Science and Technology Project of Jiangxi Provincal Department of Education (GJJ20151118), the National Natural Science Foundation of China (31460149), the Key Research Project of Jiangxi Provincal Department of Science and Technology (20161BBG70050), the Student Training Project of Nanchang Institute of Technology in 2016
通讯作者:
田胜尼
E-mail: tian7173@163.com
作者简介: ZHOU Ji-hai, (1973—)，Ph. D., associate professor, Nanchang Institute of Technology e-mail:
zhoujihai2006@163.com
引用本文:
周际海，黄荣霞，袁颖红，田胜尼，胡 良，程 坤，唐嘉婕，张文锋. 超声萃取-酶标仪-微量法测定污染土壤中的总石油烃[J]. 光谱学与光谱分析, 2017, 37(11): 3647-3652.
ZHOU Ji-hai, HUANG Rong-xia, YUAN Ying-hong, TIAN Sheng-ni, HU Liang, CHENG Kun, TANG Jia-jie, ZHANG Wen-feng. Microtitration Assay of Total Petroleum Hydrocarbons in Contaminated Soils Using Ultrasonic Extraction and Multiskan Spectrum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3647-3652.
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