1. 西北大学文化遗产学院，陕西 西安 710069
2. 文化遗产研究与保护技术教育部重点实验室，陕西 西安 710069
3. Institute of Conservation and Restoration，University of Applied Arts Vienna，Vienna 1010，Austria
Study on Secondary Structural Changes of Proteinaceous Binding Media in Ancient Polychromy Artwork after Light Aging by FTIR Spectroscopy
MA Zhen-zhen1, 2，WANG Li-qin1, 2*，Gabriela Krist3，Tatjana Bayerova3，YANG Lu1, 2
1. School of Cultural Heritage, Northwest University, Xi’an 710069，China
2. Key Laboratory of Cultural Heritage Research and Conservation of Ministry of Education, Northwest University, Xi’an 710069，China
3. Institute of Conservation and Restoration, University of Applied Arts Vienna, Vienna 1010，Austria
Abstract：Animal glue and egg white are important proteinaceous binding media of ancient polychromy artworks, and the research of their structural changes caused by light aging is highly related to the exploration of pathological changes mechanism as well as the selection of scientific materials and proper conservative methods. Regarding the animal glues (bone, rabbit skin, sturgeon) and egg white in Kunsthistorisches Museum Vienna as the study objects, this paper aimed at studying the protein secondary structure changes after light aging by Fourier transform infrared spectroscopy (FTIR) with the help of deconvolved Amide I and Gaussian fitting method. Results illustrated that the four binders still possessed the typical FTIR spectra characteristics after light aging in spite of some peaks shift, among which glycosidic bond in egg white showed an obvious blue shift. Moreover, deconvolved Amide I and Gaussian fitting results indicated that the contents of α-helix, parallel β-sheets, anti-parallel β-sheets, β-turns and random coils of each binder varied after the light aging. To be specific, α-helix’s content of the bone decreased 22.24% but random coils increased 9.76%, which deduced the uncoiling of the α-helix caused by light. The α-helix’s contents of the rabbit skin and sturgeon reduced 5.31% and 4. 15% respectively, and random coils content of the former showed the minimum growth (6.96%) while that of the latter decreased. Thus, these two were supposed to be of high stability versus light. Egg white showed the poorest light stability due to the highest reduction of α-helix (44.45%) and obvious rise of random coils (27.49%). In addition, the anti-parallel β-sheets’ contents of all the binders increased while the β-turns kept stable. Parallel β-sheets showed a decline trend among all the binders except the sturgeon glue. This research confirmed the feasibility of studying the proteinaceous binders’ secondary structure changes by deconvolved Amide I, Gaussian fitting method and FTIR spectroscopy, which was proved to be a promising method in studying the aging phenomenon of the proteinaceous binders in artworks. However, the transformation mechanism needs further explorations.
马珍珍，王丽琴，Gabriela Krist，Tatjana Bayerova，杨 璐. 红外光谱法研究古代彩绘蛋白胶料光老化的二级结构变化[J]. 光谱学与光谱分析, 2017, 37(09): 2712-2716.
MA Zhen-zhen，WANG Li-qin，Gabriela Krist，Tatjana Bayerova，YANG Lu. Study on Secondary Structural Changes of Proteinaceous Binding Media in Ancient Polychromy Artwork after Light Aging by FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(09): 2712-2716.
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