The Effect of Clove on the Variation of Fluorescence Characteristic during Low Density Lipoprotein Oxidation
ZENG Jun-jie1, JIANG Shen-hua2,3*, FENG Hua1, SU Guo-xing1*
1. School of Basic Medicine and Biology, Soochow University, Suzhou 215123, China
2. School of Pharmacy and Life Science, Jiujiang University, Jiujiang 332000, China
3. Jiujiang Andehe Biotechnology Co., Ltd., Jiujiang 332000, China
Abstract：Oxidation of LDL has been regarded as the key factor which leads to AS, the fluorescent characteristic of LDL developed a series of changes during LDL oxidation. The traditional and three-dimensional flourescence contour spectroscopy were used to evaluate the inhibition effects of clove against the changes of fluorescent characteristic of LDL in the process of oxidation in this paper. The results indicated as follows: the optimal measurement time point of LDL incubation for fluorescence was 48 h; fluorescence of Trp quenching, fluorescent chromophore redshift, Lys residues contained in apoB-100 being modified by reactive aldehydes generated during oxidation, the generation of fluorescent materials and lipofuscins and the variation of three-dimensional fluorescence were significantly inhibited, respectively, and the inhibition effects were positively related with concentrations of clove crude extracts. Moreover, in this experiment it was found that the different polarity components of clove (n-hexane fraction (clove essential oil), ethyl acetate fraction, n-butyl alcohol fraction and water fraction) possessed different activities on influencing the changes of fluorescent characteristic of LDL during LDL oxidation. Among these fractions, n-hexane fraction possessed the strongest activities against Trp fluorescence quenching, fluorescent chromophore redshift and the variation of three-dimensional fluorescence. However, the inhibition efficiencies of ethyl acetate fraction were the highest against the generation of fluorescent materials and lipofuscins, Lys residues being modified by reactive aldehydes. The results suggest that clove has the preferable inhibitory action on the variation of fluorescence characteristic during LDL oxidation, and the main components of inhibition focus on the essential oil and ethyl acetate fraction. This research provides a reference on further components analysis, research and development of functional foods of clove.
曾钧杰，江慎华，丰 华，苏国兴. 丁香对低密度脂蛋白氧化过程中荧光特性变化的影响[J]. 光谱学与光谱分析, 2017, 37(08): 2452-2461.
ZENG Jun-jie, JIANG Shen-hua, FENG Hua, SU Guo-xing. The Effect of Clove on the Variation of Fluorescence Characteristic during Low Density Lipoprotein Oxidation. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(08): 2452-2461.
[1] Thilakarathnasurangi H, Vasantha R P. Canadian Journal of Plant Science, 2012, 92(3): 407.
[2] Murtola T, Vuorela T A, Hyvnen M T, et al. Soft Matter, 2011, 7(18): 8135.
[3] Itabe H. Progress in Lipid Research, 1998, 37(2-3): 181.
[4] Levitan I, Volkov S, Subbaiah P V. Antioxidants and Redox Signalling, 2010, 13(1): 39.
[5] YANG Qiong-yu, WAN Yan, JIANG Shen-hua, et al(杨琼玉，万 严，江慎华，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2017，37(1)： 312.
[6] Cominacini L, Garbin U, Davoli A, et al. Journal of Lipid Research, 1991, 32(2): 349.
[7] Koller E, Quehenberger O, Jürgens G, et al. FEBS Letters, 1986, 198(2): 229.
[8] Horakova L, Giessauf A, Raber G, et al. Biochemical Pharmacology, 1996, 51(10): 1277.
[9] Esterbauer H，Gebicki J，Puhl H，et al. Free Radical Biology & Medicine, 1992, 13(4): 341.
[10] Karten B, Beisiegel U, Gercken G, et al. Chemistry & Physics of Lipids, 1997, 88(2): 83.
[11] McLean L R, Hagaman K A. Biochemistry, 1989, 28(1): 321.
[12] Picard S, Parthasarathy S, Fruebis J, et al. Proceedings of the National Academy of Science of the United States of America, 1992, 89(15): 6876.
[13] Chen Z, Liu Q, Wang S, et al. Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy, 1999, 55(9): 1909.
[14] Kumaran R, Ramamurthy P. Journal of Luminescence, 2014, 148(7): 277.
[15] DAN Chun, FU Tie-jun, LIU Zhong-rong, et al(但 春，付铁军，刘忠荣，等). Journal of Instrumental Analysis(分析测试学报), 2004, 23: 87.
[16] Charles D J. Antioxidant Properties of Spices, Herbs and Other Sources. New York: Springer New York Heidelberg Dordrecht London，2013, chapter 20： 245.
[17] JIANG Shen-hua, XIAO Min, JIANG Chun-xia, et al(江慎华，肖 敏，江春霞，等). Modern Food Science and Technology(现代食品科技), 2013, (9): 2063.
[18] Jiang Shenhua, Li Hanquan, Ma Haile, et al. International Journal of Food Sciences and Nutrition, 2011, 62(5): 441.
[19] Chen X, Ren L, Li M, et al. Food Chemistry, 2017, 214: 432.
[20] SHEN Yong-gen, CAI Zhi-peng, JIANG Shen-hua, et al(沈勇根，蔡志鹏，江慎华，等). Transactions of the Chinese Society for Agricultural Machinery(农业机械学报), 2012, 43(10): 131.
[21] Jin S, Cho K H. Food and Chemical Toxicology, 2011, 49(7): 1521.
[22] Wieland H, Seidel D. Journal of Lipid Research, 1983, 24(7): 904.
[23] Chen C Y, Milbury P E, Chung S K, et al. Journal of Nutritional Biochemistry, 2007, 18(12): 785.
[24] Cristobal L Miranda, Jan F Stevens, Vadim Ivanov, et al. Journal of Agricultural and Food Chemistry, 2000, 48(9): 3876.
[25] Ilya P, Dov L. Chemistry and Physics of Lipids, 2014, 178(11): 63.
[26] Gieβauf A, Wickern B V, Simat T, et al. FEBS Letters, 1996, 389(2): 136.
[27] Itakura K, Oya Ito-T, Osawa T, et al. FEBS Letters, 2000, 473(2): 249.
[28] Sicchieri L B, Monteiro A M, Samad R E, et al. Applied Spectroscopy, 2013, 67(4): 379.
