Feasibility Study of Dividing Maize Heterotic Group Based on Near Infrared Spectroscopy
JIA Shi-qiang1, LI Hao-chuan2, AN Dong1,3*, LIU Zong-hua2*
1. College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China
2. College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, China
3. Key Laboratory of Agricultural Information Acquisition Technology (Beijing), Ministry of Agriculture, Beijing 100083, China
Abstract：Accurate division of heterotic group in maize can provide effective information on germplasm improvement, heterosis mode construction, and development of new varieties. The main methods used at present to divide maize heterotic group are pedigree, combining ability test, isozyme markers, and molecular markers. These methods are inferior because of their high cost and operational complexity. Some of them even have to destroy the seeds. This study explored novel method feasibility of near infrared (NIR) spectroscopy in quick nondestructive heterotic grouping of maize. The near infrared diffuse reflection spectra of maize seeds were collected and preprocessed using smoothing, first derivative, and vector normalization. The features of these spectra were extracted through principal component analysis (PCA). Twelve Chinese maize inbred line samples were selected, including six leading inbred lines (Part A) and six excellent self-selection lines (Part B). Part A was divided into three groups with NIR spectroscopy, namely, A1 (Zheng58) and A2 (Ye478), A3 (Chang7-2) and A4 (Huangzaosi), and A5 (Mo17) and A6 (SiF1). This division was in accordance with pedigree analysis. Part B was also divided into three groups by NIR spectroscopy: B1 and B2, B3 and B4, as well as B5 and B6. This division conforms to clustering analysis based on SSR molecular marker. These processes confirm that NIR spectroscopy is a convenient, highly efficient, and feasible heterotic grouping method of maize.
基金资助: China Agricultural University Education Foundation Dabeinong Education Fund (1081-2413001), National Special Fund for Agro-scientific Research in the Public Interest (201203052), National 863 project (2012AA101104-3)
作者简介: JIA Shi-qiang, (1989—), Master Student, China Agricultural University
LI Hao-chuan, (1978—), Associate Professor, Henan Agricultural University
JIA Shi-qiang and LI Hao-chuan：joint first authors
引用本文:
贾仕强，李浩川，安 冬，刘宗华. 利用近红外光谱进行玉米杂种优势类群划分的可行性研究[J]. 光谱学与光谱分析, 2017, 37(05): 1634-1641.
JIA Shi-qiang, LI Hao-chuan, AN Dong, LIU Zong-hua. Feasibility Study of Dividing Maize Heterotic Group Based on Near Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1634-1641.
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