FTIR Spectroscopic Characterization of Material Composition and Structure of Leaves of Different Citrus Rootstocks under Boron Stress
LU Xiao-pei, JIANG Cun-cang*, DONG Xiao-chang, WU Xiu-wen, YAN Lei
Microelement Research Center, Huazhong Agricultural University/Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtse River), Ministry of Agriculture, Wuhan 430070, China
Abstract:Boron (B) is an essential trace element for higher plants, the threshold between deficiency and toxicity is narrow, so the problems of yield and quality of crops due to B deficiency and B toxicity have been attracting great attention home and abroad. To investigate the effects on the component and structure changes in leaf of two kinds of citrus rootstocks (citrange and trifoliate orange) under B stress situations by using Fourier transform infrared (FTIR) spectroscopy. The results showed that: (1) the growth of citrus rootstock was inhibited by the low B and high B stress situations. Symptoms of B-toxicity were first appeared in old leaves as tip yellowing in trifoliate orange, in contrast, almost no visible symptoms occurred in citrange, suggesting that citrange was more tolerant to B-toxicity than trifoliate orange. (2) the characteristic peaks were at 1 153, 1 053 and 1 028 cm-1 disappeared due to low B stress in trifoliate orange, but only 1 055 cm-1 disappeared in citrange, and relative absorbance of other peaks was increased compared with control, suggesting that the structure of cellulose glycosides, soluble sugar and ribosome in leaf changed and other components increased under low B stress in trifoliate orange, only soluble sugar changed in citrange ones. Thus, the effect of low B stress on trifoliate orange is higher than that of citrange. (3) the characteristic peaks at 1 153, 1 053 cm-1 disappeared due to high B stress in trifoliate orange, however, 1 024 cm-1 disappeared in citrange, the relative absorbance of other peaks was reduced compared with control, indicated that the structure of protein and soluble sugar in leaf changed and other compositions reduced under high B stress in trifoliate orange, and ribosome changed in citrange ones. Therefore, the effect of high B stress on trifoliate orange is higher than that of citrange in polysaccharide structure. Results show that citrange was more tolerant to low B and high B stress than trifoliate orange, and one of the key factors contributing to the result is the differences of B effect on chemical composition and structure between two rootstocks.
Key words:Boron stress; Citrus rootstock; FTIR; Material composition and structure
卢晓佩,姜存仓,董肖昌,吴秀文,闫 磊. 硼胁迫下不同柑橘砧木叶片物质组成及结构的FTIR表征[J]. 光谱学与光谱分析, 2017, 37(05): 1380-1385.
LU Xiao-pei, JIANG Cun-cang, DONG Xiao-chang, WU Xiu-wen, YAN Lei. FTIR Spectroscopic Characterization of Material Composition and Structure of Leaves of Different Citrus Rootstocks under Boron Stress. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1380-1385.
[1] Liu Guidong, Wang Ruidong, Liu Leichao, et al. Plant and Soil, 2013, 370: 555.
[2] JIAO Xiao-yan, WANG Jin-song, WU Ai-lian, et al(焦晓燕, 王劲松, 武爱莲, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2013, 19(3): 615.
[3] Pande N, Yarchan A. The Journal of the Indian Botanical Society, 2011, 3: 56.
[4] WANG Rui-dong, JIANG Cun-cang, LIU Gui-dong, et al(王瑞东, 姜存仓, 刘桂东, 等). Chinese Agricultural Science Bulletin(中国农学通报), 2011, 27(18): 12.
[5] JIANG Cun-cang, WANG Yun-hua, LIU Gui-dong, et al(姜存仓, 王运华, 刘桂东, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2009, 15(3): 656.
[6] Robert J Reid. Spring New York, 2013, 333.
[7] PAN Guo-ping(潘国平). News of Citrus and Subtropical Fruit(柑橘与亚热带果树信息), 2004, 20(2): 42.
[8] WANG Cheng, JIANG Ze-ping, LI Wen-qing, et al(王 成, 蒋泽平, 李文青, 等). Journal of Agro-Environment Science(中国环境科学学报), 2014, 33(4): 673.
[9] Papageorgiou S K, Kouvelos E P, Favvas E P, et al. Carbohydrate Research, 2010, 345(4):469.
[10] XUE Sheng-guo, ZHU Feng, YE Chen, et al(薛生国, 朱 锋, 叶 晨, 等). Acta Ecologica Sinica(生态学报), 2011, 31(20): 6143.
[11] GONG Ning, LI Rong-hua, MENG Zhao-fu, et al(龚 宁, 李荣华, 孟昭福, 等). Journal of Agro-Environment Science(农业环境科学学报),2010, 29(1): 9.
[12] FU Chuan, YU Shun-hui, HUANG Yi-min, et al(付 川, 余顺慧, 黄怡民, 等). Acta Ecologica Sinica(生态学报), 2014, 34(5): 1149.
[13] WANG Sheng-feng, LIU Yun-xia, GAO Li-li, et al(王盛锋, 刘云霞, 高丽丽, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2014, 20(4): 1005.
[14] Liu Guidong, Dong Xiaochang, Liu Leichao, et al. Scientia Horticulturae, 2014, 176: 54.
[15] Yang J, Yen H Y. Plant Physiol., 2002, 130: 1032.
[16] Christian Z, Mehmet S, Edgar P. Journal of Plant Physiology, 2014, (171): 656.
[17] WU Xiu-wen, HAO Yan-shu, LEI Jing, et al(吴秀文, 郝艳淑, 雷 晶, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2016, 36(3): 676.
[18] WANG Zhen-yu, ZHANG Fu-suo(王震宇, 张福锁). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 1995, 1(1): 52.
[19] Papadakis I E, Dimassi K N, Bosabalidis A M, et al. Plant Science, 2004, 166(2): 539.