Research on Molecular Spectra of Interactions Between Salvianolic Acid A and Salvianolic Acid B with Insulin, and Effect of Glucose on the Binding
YANG Wen-yue1, CUI Lin1, QU Ling-ling1, XU Ning1, HUANG Yun1,2*, CUI Li-jian3*, ZHAN Wen-hong1, ZHAO Ding1
1. Pharmaceutical College, Hebei Medical University, Shijiazhuang 050017, China 2. Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang 050017, China 3. Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang 050091, China
Abstract：The interactions of Salvianolic acid A (SAA) and Salvianolic acid B (SAB) with insulin were studied by using fluorescence spectroscopy, UV-vis spectroscopy and ATR-FTIR spectroscopy in simulating physiological condition (pH 7.40). The fluorescence quenching of insulin by SAA and SAB were static quenching process. The results of synchronous fluorescence and three-dimensional fluorescence spectra suggested no obvious conformation changes of insulin after SAA or SAB binding. But ATR-FTIR spectra showed that SAA and SAB could change the secondary structures of insulin, of which β-turns decreased and random coil increased accompanied with α-helices and β-sheets no clear change. The glucose might influenced the the bioactivity of insulin in the SAA-insulin and SAB-insulin systems by changing the binding constants of SAA (or SAB) with insulin and exacerbating the changes of insulin conformation and relative contents of α-helices.
杨文月1，崔 琳1，渠玲玲1，许 宁1，黄 芸1,2*，崔力剑3*，詹文红1，赵 丁1 . 丹参酚酸A和丹参酚酸B与胰岛素相互作用的分子光谱学研究以及葡萄糖的影响 [J]. 光谱学与光谱分析, 2016, 36(09): 3053-3061.
YANG Wen-yue1, CUI Lin1, QU Ling-ling1, XU Ning1, HUANG Yun1,2*, CUI Li-jian3*, ZHAN Wen-hong1, ZHAO Ding1. Research on Molecular Spectra of Interactions Between Salvianolic Acid A and Salvianolic Acid B with Insulin, and Effect of Glucose on the Binding . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(09): 3053-3061.
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