Abstract:Phytoremediation is a novel method with great potential for site remediation contaminated by heavy metals in future. The cell wall of plant roots is significant to affect the remediation efficiency, for it is related to the multi-interface of heavy metals, pedosphere and plant. The relationship between cell wall of plant and heavy metals is complicated, containing the reaction behavior of physical chemistry, physiology and biochemistry. At present, the spectral technologies are not adequately used to investigate the in-situ response characteristics between cell wall of plant roots and heavy metals. The Calendula officinalis seedlings, the remediation plant in loess, were used as experimental sampleswhile the root characteristic variation was revealed on cell wall. The approaches of X-ray fluorescence (XRF), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Raman spectra were applied to analyze the response effect of cell wall to lead/cadmium stress. The results showed: the cell wall appears to be shrinking, and certain amounts of dark particles appear on cell wall. The contents of lead/cadmium increase greatly as shown from XRF, while the representative crystals of lead/cadmium are hardly detected. The absorbance peak at 3 416 cm-1 indicates the coordination effect between lead/cadmium and —OH in FTIR, and the movement of absorbance peaks, from 1 701 to 1 736 cm-1 and 1 593 to 1 618 cm-1, respectively, indicates the different characteristics of protein in cell wall of Calendula officinalis seedlings roots under lead/cadmium stress. The Raman intensity of absorbance peak at 2 960 cm-1 increases under lead/cadmium stress, and it proves the changes on arranging directions of cellulose molecules in cell wall samples. The components (Pectin, protein, cellulose, etc.) and functional groups (—OH, N—H, CO, etc.) of cell wall play an important role in the resistance process of cell wall derived from Calendula officinalis seedlings roots to the stress of lead/cadmium in loess.
范春辉,高雅琳,杜 波 . 黄土区金盏菊幼苗根部细胞壁对Pb/Cd复合胁迫响应的FTIR和Raman光谱 [J]. 光谱学与光谱分析, 2016, 36(07): 2076-2081.
FAN Chun-hui, GAO Ya-lin, DU Bo . Response of FTIR and Raman Spectra on Cell Wall of Calendula Officinalis Seedlings Roots to the Co-Contamination Stress of Lead and Cadmium in Loess. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2076-2081.
[1] Ali H, Khan E, Sajad M A. Chemosphere, 2013, 91: 869. [2] Ullah A, Heng S, Munis M F H, et al. Environmental and Experimental Botany, 2015, 117: 28. [3] Brady D, Duncan J R. Enzyme and Microbial Technology, 1994, 16: 633. [4] Fernández R, Fernández-Fuego D, Bertrand A, et al. Plant Physiology and Biochemistry, 2014, 78: 63. [5] Vuletic M, Hadzi-Taskovic S V, Markovic K, et al. Plant Biology, 2014, 16: 88. [6] Konno H, Nakashima S, Katoh K. Journal of Plant Physiology, 2010, 167: 358. [7] Akyuz T, Akyuz S, Gulec A. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, 149: 744. [8] Nishizono H, Ichikawa H, Suziki S, et al. Plant and Soil, 1987, 101: 15. [9] Rodríguez P, Munoz-Aguirre N, San-Martin M E, et al. Applied Surface Science, 2008, 255: 740. [10] Fadavi G, Mohammadifar M A, Zargarran A, et al. Carbohydrate Polymers, 2014, 101: 1074. [11] Iqbal M, Saeed A, Zafar S I. Journal of Hazardous Materials, 2009, 164: 161. [12] Wilson C J, Apiyo D, Wittung-Stafshede P. Quarterly Reviews of Biophysics, 2004, 37: 285. [13] Richter S, Müssig J, Gierlinger N. Planta, 2011, 233: 763. [14] Edwards H G M, Farwell D W, Webster D. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 1997, 53: 2383. [15] Himmelsbach D S, Akin D E. Journal of Agricultural and Food Chemistry, 1998, 46: 991. [16] Martinez-Sanz M, Gidley M J, Gilbert E P. Carbohydrate Polymers, 2015, 125: 120.