Effect of Four Types of Chemical Pretreatment on Enzymatic Hydrolysis by SEM, XRD and FTIR Analysis
JIN Shu-guang1, ZHANG Guang-ming2, ZHANG Pan-yue1*, ZHOU Jin-chi3, GAO Yong-wei3, SHI Jun-na3
1. Beijing Key Lab for Source Control Technology of Water Pollution, Beijing Forestry University, Beijing 100083, China 2. School of Environment and Resource, Renmin University of China, Beijing 100872, China 3. Analysis Test Center, Beijing Forestry University, Beijing 100083, China
Abstract：Catalpa sawdust was respectively pretreated by NaOH, Ca(OH)2, H2SO4 and HCl solution, and the enzymatic hydrolysis of catalpa sawdust was significantly enhanced by alkaline pretreatments. In order to investigate the mechanisms of pretreatment of catalpa sawdust, the characteristics of catalpa sawdust before and after pretreatments were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. It was found that the surface of catalpa sawdust was disrupted by four kinds of chemical pretreatment, and the pretreatment with Ca(OH)2 solution resulted in the most serious damage. The XRD results showed that part of amorphous regions was damaged by alkaline pretreatments, which led to a relative increase of crystallinity Index (CrI) of catalpa sawdust; while the CrI of catalpa sawdust was insignificantly influenced by acid pretreatments. The FTIR analysis displayed that the molecular structures of hemicellulose and lignin of catalpa sawdust were damaged in different degrees by four types of pretreatment. The significant improvement of enzymatic hydrolysis of catalpa sawdust after alkaline pretreatment might be attributed to the effective delignification of alkaline.
金曙光1, 张光明2, 张盼月1*, 周金池3, 高永伟3, 史军娜3. 利用SEM, XRD和FTIR研究四种化学预处理对楸木木屑酶解的影响[J]. 光谱学与光谱分析, 2016, 36(06): 1966-1970.
JIN Shu-guang1, ZHANG Guang-ming2, ZHANG Pan-yue1*, ZHOU Jin-chi3, GAO Yong-wei3, SHI Jun-na3. Effect of Four Types of Chemical Pretreatment on Enzymatic Hydrolysis by SEM, XRD and FTIR Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(06): 1966-1970.
