Determination of Benzo(a)pyrene in Cigarette Mainstream Smoke by Second-Order-Derivative Synchronous Fluorescence
FAN Hu1, 2,SHENG Liang-quan1, TONG Hong-wu1, JIN Zhong-xiu1, 2,LIU Shao-min1*
1. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, China 2. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
Abstract：The synchronous fluorescence, first-order and second-order-derivative synchronous fluorescence spectra of BaP in cigarette mainstream smoke were studied. It was indicated that the second-order-derivative synchronous fluorescence spectrum could decrease the disturbances of BaP homologous compounds and background of sample. Hereby the present paper introduces a new method to determine BaP in cigarette mainstream smoke by solid-phase-extraction second-order-derivative synchronous fluorescence. The detection limit is 0.2 ng·mL-1, the average recoveries of BaP are 80.2%-86.2%, and the RSD is 2.64%-3.02%.
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