Application of Near-Infrared Diffuse Reflectance Spectroscopy to the Detection and Identification of Transgenic Corn
RUI Yu-kui1, LUO Yun-bo1, HUANG Kun-lun1, WANG Wei-min2, ZHANG Lu-da3*
1. Laboratory of Food Technology of China Agricultural University, Beijing 100094, China 2. Center of Science and Technology Development, Ministry of Agricultural, Beijing 100026, China 3. Science College of China Agricultural University, Beijing 100094, China
Abstract：With the rapid development of the GMO, more and more GMO food has been pouring into the market. Much attention has been paid to GMO labeling under the controversy of GMO safety. Transgenic corns and their parents were scanned by continuous wave of near infrared diffuse reflectance spectroscopy range of 12 000-4 000 cm-1; the resolution was 4 cm-1; scanning was carried out for 64 times; BP algorithm was applied for data processing. The GMO food was easily resolved. Near-infrared diffuse reflectance spectroscopy is unpolluted and inexpensive compared with PCR and ELISA, so it is a very promising detection method for GMO food.
Key words：Near-infrared spectroscopy;Transgenic food safety;Detection and identification
芮玉奎1，罗云波1，黄昆仑1，王为民2，张录达3* . 近红外光谱在转基因玉米检测识别中的应用[J]. 光谱学与光谱分析, 2005, 25(10): 1581-1583.
RUI Yu-kui1, LUO Yun-bo1, HUANG Kun-lun1, WANG Wei-min2, ZHANG Lu-da3* . Application of Near-Infrared Diffuse Reflectance Spectroscopy to the Detection and Identification of Transgenic Corn. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1581-1583.
[1] CHEN Yong-hong, LI Zhe-min, XU Shi-wei(陈永红，李哲敏，许世卫). Food and Nutrition in China(中国食物与营养), 2003, (9): 4. [2] Hellebrand M. Z Lebensm Unters Forsch A, 1998, 206: 83. [3] Rogan G J, Dudin Y A, Lee T C, et al. Food Control, 1999, 10(6): 407. [4] XU Guang-tong, YUAN Hong-fu, LU Wan-zhen(徐广通，袁洪福，陆婉珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000，20(2)：134. [5] WANG Duo-jia, ZHOU Xiang-yang, JIN Tong-ming, HU Xiang-na, ZHONG Jiao-e, WU Qi-tang(王多加，周向阳，金同铭，胡祥娜，钟娇娥，吴启堂). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(4)：447. [6] Marvik O. Process Control and Quality, 1997, 9(4): 127. [7] Johnsen E. Process Control and Quality, 1997, 9(4): 205. [8] Meurens M, Li W J, Foulon M, et al. Cerevisia, 1995, 20(3): 33. [9] QI Xiao-ming, ZHANG Lu-da, DU Xiao-lin, SONG Zhao-juan, ZHANG Yi, XU Shu-yan(齐小明，张录达，杜晓林，宋昭娟，张 一，徐淑燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(5): 870. [10] ZHOU Ping-ping, WU Yong-ning, ZHANG Jian-zhong(周萍萍 吴永宁 张建中). Chinese Journal of Food Hygiene(中国食品卫生杂志), 2004, 16(3): 254. [11] Lü Shan-hua, QIU Li-juan, TAO Bo(吕山花，邱丽娟，陶 波). Biotechnology Bulletin(生物技术通报), 2002，(4)：34.
