Abstract:Propolis possesses functions of antibacterial, antiviral, anticancer, and liver protection, and is known as the “purple gold”, however, the phenomenon which making and selling of counterfeit are growing in intensity. In order to establish a authenticity and quality of propolis evaluation model, in this paper, forty-one Chinese propolis, one proplis from United States and two tree gums were used for experimental materials. The infrared spectrum collection was performed by Fourier transform infrared spectrometer, and principal component analysis (PCA) was used for data analysis. The result showed that, the infrared spectrum of propolis and tree gum were significantly different. The propolis characteristic peak only appeared in 2 500~3 500 and 400~1 800 cm-1. All propolis had two frequency region of characteristic peaks, 2 849.08~2 848.53 and 2 917.74~2 916.76 cm-1, but tree gum did not have characteristic peak in this region. The characteristic peaks of gum were in 1 150~1 300 and 1 550~1 650 cm-1. Differences in these aspects can be used to distinguish propolis and gum, and can be used to identify true and false propolis. We use Qinghai propolis as a standard sample, in 42 samples, the matching degree of other propolis is >80%. In addition, the result of PCA shows that tree gum and the propolis from different climate zone, or with different colors could be distinguished well. This paper firstly performed analysis on different propolis and gum by infrared spectrum, and a new method, for authenticity and quality of propolis identification, could be developed.
罗火林,刘星星,龚上佶,郭夏丽,罗丽萍* . 不同地区蜂胶和树胶的红外谱图分析 [J]. 光谱学与光谱分析, 2015, 35(11): 3058-3062.
LUO Huo-lin, LIU Xing-xing, GONG Shang-ji, GUO Xia-li, LUO Li-ping* . Infrared Spectrum Analysis of Propolis and Tree Gum Collected from Different Areas. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(11): 3058-3062.
[1] Zhang C P, Zheng H Q, Hu F L. J. Food Sci., 2011, 76(1): 75. [2] Hegazi A G, Hady F K, Shaker K H, et al. Mellifera, 2012, 12(23): 2. [3] Turan I, Demir S, Misir S, et al. Tropical Journal of Pharmaceutica Rerearch, 2015, 14(5): 777. [4] Zizic J B, Vukovic N L, Jadranin M B, et al. J. Sci. Food Agric., 2013, 93(12): 3001. [5] WANG Shan-shan, WANG Jie, YANG Wen-chao, et al(王姗姗, 王 捷, 杨文超,等). Apicultural of China(中国蜂业), 2012, 63(3): 74. [6] Inui S, Hosoya T, Kumazaw S. Natural Product Communications, 2014, 9(2): 165. [7] Zhang Cuiping, Huang Shuai, Wei Wenting, et al. J. Food Sci., 2014, 79(7): C1315. [8] Palacio S, Aitkenhead M, Escudero A, et al. PLoS One, 2014, (9): e107285. [9] Saltas D, Pappas C S, Daferera D, et al. J. Agric. Food Chem., 2013, 61(13): 3235. [10] Kurz C, Leitenberger M, Carle R, et al. Food Chemistry, 2010, 119(2), 806. [11] Yang J, Qin Y, Yuan M, et al. Int. J. Biol. Macromol., 2013, 62: 411. [12] Neo Y P, Swift S, Ray S, et al. Food Chem., 2013, 141(3): 3192. [13] Wang J, Huang H H, Cheng Y F, et al. J. Med. Food, 2012, 15(10): 930. [14] Wu Y W, Sun S Q, Zhao J, et al. Journal of Molecular Structure, 2008, 883(2): 48. [15] Guo X, Chen B, Luo L, et al. J. Agric. Food Chem., 2011, 59(23): 12610. [16] Gong S, Luo L, Gong W, et al. Food Chemistry, 2012, 134(1): 583.