Application of SDS Micellar Solution in Fluorescence Spectral Measurement of Petroleum Pollutant
YANG Li-li1, WANG Yu-tian1, LU Xin-qiong2
1. Key Laboratory of Measurement Technology and Instrumentation of Hebei Province, Yanshan University, Qinhuangdao 066004, China 2. State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing 100084, China
Abstract:A new method of solubilizing, sensitizing and stabilizing petroleum substances by using sodium dodecyl sulfate (SDS) micellar solution as solvent was proposed. The variation relationship between fluorescence intensity of petroleum substances and SDS micellar solution concentration was studied, and the optimum concentration of SDS micelle solution as solvent was determined with 0.1 mol·L-1. Gasoline, diesel and kerosene SDS micellar solution of different diluted concentration were measured using FLS920 fluorescence spectrometer and fluorescence excitation-emission matrixes (EEMs) were obtained. After analyzing Rayleigh scattering, Raman scattering and instrument characteristics influence on measured spectrum, three-dimensional fluorescence spectra of three kinds of oil samples with excitation wavelength ranging from 250 to 400 nm and emission wavelength from 260 to 500 nm were established by spectral correction. The linear relationship between fluorescence intensity and concentration in certain concentration range was determined. By comparison with the spectra of gasoline, diesel, kerosene aqueous solution with the various concentrations under the same conditions and preparation method, the solubility and fluorescence intensity of petroleum pollutant in water was increased, and better stability was obtained. The measurement of petroleum substances without relying on some toxic solvent extraction was not only achieved, but its low solubility in water and difficult quantitative problems were solved.
杨丽丽1,王玉田1,鲁信琼2 . SDS胶束溶液在石油类污染物荧光光谱测量中的应用 [J]. 光谱学与光谱分析, 2015, 35(06): 1618-1623.
YANG Li-li1, WANG Yu-tian1, LU Xin-qiong2 . Application of SDS Micellar Solution in Fluorescence Spectral Measurement of Petroleum Pollutant. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(06): 1618-1623.
[1] Aguilera F, Méndez J, Pásaro E, et al. Journal of Applied Toxicology, 2010, 30(4): 291. [2] Rogowska J, Namiesnik J. Environmental Implications of Oil Spills Fromshipping Accidents. New York: Springer, 2010. 95. [3] GAO Zhen-hui, YANG Jian-qiang, WANG Pei-gang, et al(高振会, 杨建强, 王培刚, 等). Theory, Methods and Case Studies of Ecological Damage Assessment from Marine Oil Spill(海洋溢油生态损害评估的理论、方法及案例研究). Beijing: Ocean Press(北京: 海洋出版社), 2007. 4. [4] XU Jin-gou, WANG Zun-ben(许金钩, 王尊本). Method of Fluorescence Analysis(荧光分析法). Beijing: Science Press(北京: 科学出版社), 2006. 11. [5] Lázaro E, San Andrés M P, Vera S. Analytica Chimica Acta, 2000, 413(1): 159. [6] Ocana J A, Barragan F J, Callejon M. Journal of Pharmaceutiacl and Biomedical Analysis, 2005, 37(2): 327. [7] Saitoh T, Itoh H, Hiraide M. Talanta, 2009, 79(2): 177. [8] Morales T V, Esponda S M, Rodríguez J J S, et al. Macedonian Journal of Chemistry and Chemical Engineering, 2010, 29(1): 1. [9] Sales P S, de Rossi R H, Fernández M A. Chemosphere, 2011, 84(11): 1700. [10] HUANG Hui, LI Li, MA Qiao, et al(黄 晖, 李 丽, 马 乔, 等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(2): 249. [11] FENG Yong-lan, ZHOU Que(冯泳兰, 周 雀). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2009, 26(5): 1260. [12] WANG Han, CHI Yan-hua, SHANG Li-ping, et al(王 晗, 迟燕华, 尚丽平, 等). Physical Testing and Chemical Analysis·Chemical Analysis(理化检验·化学分册), 2010, 46(7): 803.