1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China 2. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 3. Agro-Environmental Protection Institute of Ministry of Agriculture，Tianjin 300191，China
Abstract：In the present paper, the analysis of the content of CP, NDF and ADF in the whole dry hay of oats was carried out by using near infrared reflectance spectroscopy (NIRS) technique, and in combination with the partial least square (PLS) regression algorithm the calibration analysis was performed at the same time . The results showed that the calibration models developed by the spectral data pretreatment of the second derivative+Norris smoothing, the multivariate scattering correction+second derivative+Norris smoothing, and the multivariate scattering correction were the best for CP, NDF and ADF with the same spectral regions (9 668-4 518, 9 550-5 543, 8 943-4 042 cm-1). A1l these models yielded coefficients of determination of calibration (r2cal) for CP and NDF that are both higher than 0.95, and each error lower than 3%, approached the chemical analysis precision. Moreover, the values of (RPD) of CP and NDF were both higher than 3.0. The results of these studies indicate that the contents of CP and NDF can be used to measure various samples in screening and evaluating quality constituents of dry hay in oats. While the effect of ADF modelling was poorer, the coefficients of determination of calibration (r2cal) and cross validation (r2CV)for ADF were 0.912 0, 0.855 3 respectively. The root mean square error of calibration，root mean square error of cross validation，and root mean square error of prediction ( RMSEE，RMSECV and RMSEP) for ADF were 2.33%, 2.62% and 1.91% respectively, and the precision is near the precision of the chemical analysis. The models of ADF can be used to measure various samples in screening and evaluating quality constituents of dry hay of oats also. This study has proved that NIRS technique can be applied to detect the contents of CP, NDF and ADF in the whole dry hay of oats.
Key words：Dry hay of oats;Quality;Near infrared reflectance spectroscopy(NIRS)
[1] WU Gao-lin(武高林). Forage Grass and Feed(牧草与饲料)，2007，1(1)：10. [2] LIU Gang, ZHAO Gui-qin(刘 刚，赵桂琴). Pratacultural Science, 2006, 23(11): 41. [3] Eduardo Guillin, Bermard R, Subbaiah Mechanoda. Canadian Journal of Plant Science, 1998, 78: 615. [4] MA Chun-hui, HAN Jian-guo, LI Hong-xiang, et al(马春晖，韩建国，李鸿祥，等). Acta Agrestia Sinica(草地学报)，1999，7(1)：62. [5] Tichy F, Palik S. Rostlinna Vyroba, 1994, 40(4): 359. [6] Hgun Seuny Weon. RAD Jourmal of Agricultural Science, Soil & Fertilizer, 1995, 37(2): 260. [7] BAI Qi-lin, CHEN Shao-jiang, DONG Xiao-ling, et al(白琪林，陈绍江，董晓玲，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24（11）: 1345. [8] Campbell M R, Mannis S R, Port H A, et al. Cereal Chem., 1999, 76(4): 552. [9] Association of Official Analytical Chemists，Official Methods of Analysis，15th ed. Washington，District of Colunmbia，1990，7: 27. [10] YANG Sheng(杨 胜). Analysis of Feed and Assay Methodology of Feed Quality(饲料分析及饲料质量检测技术). Beijing：Beiiing Agricultural University Press(北京：北京农业大学出版社)，1993. 58. [11] Sinnaeve G, Dardenne P, Agneessens R, et al. Journal of Near Infrared Spectrosc.，2000, 79. [12] Park R S, Agnew R E, Gordon F J，et al. Animal Feed Science and Technology, 1998, 72: 155. [13] Sinnaeve G, Dardenne P, Agneessens R, et al. Journal of Near Infrared Spectroscopy, 2000, 8: 79. [14] Park R S, Agnew R E, Gordon F J, et al. Animal Feed Science and Technology, 1998, 72: 155. [15] Frank M Sogamdares, Edward S Fry. Applied Optics, 1997, 36(33): 86. [16] Alexander J. American Society of Agronomy, 2004, 94: 316.
