Abstract：Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.
Key words：Time resolved fluorescence spectra;Fluorescein;Collision quenching;Instrument response function
潘海峰1，丁晶新1，梁荣荣1，陶占东1，刘梦薇1，张三军1*，徐建华1 . 基于猝灭荧光素的一种TCSPC仪器响应函数测量方法 [J]. 光谱学与光谱分析, 2014, 34(08): 2284-2288.
PAN Hai-feng1, DING Jing-xin1, LIANG Rong-rong1, TAO Zhan-dong1, LIU Meng-wei1, ZHANG San-jun1*, XU Jian-hua1. Quenched Fluorescein: A Reference Dye for Instrument Response Function of TCSPC. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2284-2288.
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