Identification of Dendrobium Varieties by Fourier Transform Infrared Spectroscopy Combined with Spectral Retrieval
LIU Fei1, WANG Yuan-zhong2, DENG Xing-yan3, JIN Hang2*, YANG Chun-yan1
1. Department of Physics, Yuxi Normal University, Yuxi 653100, China 2. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China 3. School of Chemistry and Biotechnology, Yunnan University of Nationalities, Kunming 650500, China
Abstract：The infrared spectral of stems of 165 trees of 23 dendrobium varieties were obtained by means of Fourier transform infrared spectroscopy technique. The spectra show that the spectra of all the samples were similar, and the main components of stem of Dendrobium is cellulose. By the spectral professional software Omnic8.0, three spectral databases were constructed. Lib01 includes of the average spectral of the first four trees of every variety, while Lib02 and Lib03 are constructed from the first-derivative spectra and the second-derivative spectra of average spectra, separately. The correlation search, the square difference retrieval and the square differential difference retrieval of the spectra are performed with the spectral database Lib01 in the specified range of 1 800～500 cm-1, and the yield correct rate of 92.7%, 74.5% and 92.7%, respectively. The square differential difference retrieval of the first-derivative spectra and the second-derivative spectra is carried out with Lib02 and Lib03 in the same specified range 1 800～500 cm-1, and shows correct rate of 93.9% for the former and 90.3% for the later. The results show that the first-derivative spectral retrieval of square differential difference algorithm is more suitabe for discerning dendrobium varieties, and FTIR combining with the spectral retrieval method can identify different varieties of Dendrobium, and the correlation retrieval, the square differential retrieval, the first-derivative spectra and second-derivative spectra retrieval in the specified spectral range are effective and simple way of distinguishing different varieties of dendrobium.
刘 飞1，王元忠2，邓星燕3，金 航2*, 杨春艳1 . 红外光谱结合光谱检索对石斛品种的鉴别研究 [J]. 光谱学与光谱分析, 2014, 34(06): 1548-1552.
LIU Fei1, WANG Yuan-zhong2, DENG Xing-yan3, JIN Hang2*, YANG Chun-yan1 . Identification of Dendrobium Varieties by Fourier Transform Infrared Spectroscopy Combined with Spectral Retrieval . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(06): 1548-1552.
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