Study on Two Different Aromas Styles of Tobacco from Guizhou by Characteristics Spectroscopic Methods
RAN Xia1, MU Lan1*, LIU Ren-xiang1, CONG Hang1, ZHANG Qing-min2, WANG Fang2
1. The Key Laboratory of Tobacco Quality Guizhou Province, Guizhou University, Guiyang 550025, China 2. Tobacco Industry Co. Ltd., of Guizhou Province, Guiyang 550001, China
Abstract：This paper made use of three-dimensional fluorescence and ultraviolet-absorption spectrum to analyze the spectral characteristics of etroleum ether extract from Guizhou flue-cured tobacco and the overall characteristic spectral information of tobacco chemical substances were obtained. The three dimensional fluorescence and ultraviolet-visible absorption spectrum of each petroleum ether extract of flue-cured tobacco from different areas are generally similar, but their intensity is different. There have three characteristic peaks in three dimensional fluorescence spectra: Ⅰ: Ex/Em=297/326 nm, Ⅱ: Ex/Em=250/330 nm, Ⅲ: Ex/Em=225/336 nm respectively and meanwhile the order of these peaks intensity is Ⅰ>Ⅲ>Ⅱ. The ultraviolet-visible absorption spectrum in 300～300 nm range presents four characteristic absorption peaks, whose maximum absorption wavelength are 329, 419, 445 and 419 nm respectively. Meanwhile, in accord with the relative intensity of characteristic peaks, it is known that there exist differences in the relative contents of the total chemical substances obtained from different flavor styles of the flue-cured tobacco. The clustering analysis results of three-dimensional fluorescence intensity score (D) and intensity ratio (R) show that in a certain range of distance coefficient, the flue-cured tobacco from different regions in Guizhou can be clearly divided into two classes “mildly sweet ”and “alcohol sweet ”. The classification can be well achieved in the smaller distance coefficient according to the ratio cluster of fluorescence intensity instead of the score cluster of fluorescence intensity. The method of three-dimensional fluorescence was better than that of ultraviolet-visible spectrometry in the matter of the clustering characteristic.
冉 霞1, 牟 兰1*, 刘仁祥1, 丛 航1, 张庆民2, 王 芳2 . 特征光谱法研究贵州两类不同香型风格烟叶 [J]. 光谱学与光谱分析, 2014, 34(03): 777-781.
RAN Xia1, MU Lan1*, LIU Ren-xiang1, CONG Hang1, ZHANG Qing-min2, WANG Fang2 . Study on Two Different Aromas Styles of Tobacco from Guizhou by Characteristics Spectroscopic Methods. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(03): 777-781.
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