Study on Experiment of Absorption Spectroscopy Detection of Pesticide Residues of Carbendazim in Orange Juice
JI Ren-dong1, 2, CHEN Meng-lan1, ZHAO Zhi-min1, 3*, ZHU Xing-yue1, WANG Le-xin1, LIU Quan-jin1，3
1. Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China 2. Huaiyin Institute of Technology, Huai’an 223003, China 3. Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing 210094, China
Abstract：Absorption spectra were studied for the carbendazim, in the mixed solution of orange juice and carbendazim using spectrophotometer. The most intensive characteristic peak (285 nm) was found in the spectrum of carbendazim standard solution. Compared with the carbendazim drug solution, the peak position of absorption spectrum has the blue shift(285～280 nm)when carbendazim(0.28 mg·mL-1)was added in the orange juice. So that we can conclude that interaction happened between the orange juice and carbendazim. Through the method of least squares fitting, the prediction models between the absorbance of orange juice and carbendazim content was obtained with a good linear relationship. The linear function model was: I=2.41+9.26x, the correlation coefficient was 0.996, and the recovery was: 81%～102%. According to the regression model, we can obtain the amount of carbendazim pesticide residues in orange juice. It was verified that the method of using ultraviolet-visible absorption spectra was feasible to detect the carbendazim residues in orange juice. The result proved that it is possible to detect pesticide residues of carbendazim in orange juice, and it can meet the needs of rapid analysis. This study provides a new way for the detection of pesticide residues.
季仁东1，2，陈梦岚1，赵志敏1，3*，朱星玥1，王乐新1，刘全金1，3 . 橙汁中多菌灵农药残留吸收光谱特征研究 [J]. 光谱学与光谱分析, 2014, 34(03): 721-724.
JI Ren-dong1, 2, CHEN Meng-lan1, ZHAO Zhi-min1, 3*, ZHU Xing-yue1, WANG Le-xin1, LIU Quan-jin1，3 . Study on Experiment of Absorption Spectroscopy Detection of Pesticide Residues of Carbendazim in Orange Juice . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(03): 721-724.
[1] Nida’ M Salem, Rafat Ahmad, Hussein Estaitieh. Chemosphere, 2009，77：673. [2] Jin Soo Park, Sun Kyoung Shin, Woo Il Kim, et al. Atmospheric Environment, 2011，45：7496. [3] JI Ren-dong，ZHAO Zhi-min，ZHANG Lin, et al(季仁东，赵志敏，张 林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2013，33(3)：668. [4] ZHAO Ke-hua, CHE Jun, HUANG An-tai, et al(赵克华，车 军，黄安太，等). Hubei Agricultural Sciences(湖北农业科学)，2010，49(5)：1193. [5] LI Jian-ke，WU Li-hua，KONG Xiang-hong(李建科，吴丽华，孔祥虹). Scientia Agricultura Sinica(中国农业科学)，2008, 41(6)：1767. [6] Jose M C, Aan V, Guillermo S M, et al. Food Chemistry, 2009, 113: 280. [7] Ting Keh-Chuh, Gerald S Tamashiro. Journal of Chromatography A, 1996，754：455. [8] Eiki Watanabe, Koji Baba, Heesoo Eun, et al. Food Chemistry, 2007, 102: 745. [9] TANG Bin(汤 斌). Based on Optical Spectroscopy for Detection of Pesticide Residues in Technology Research(基于光纤光谱技术农药残留分析测量方法研究). Chongqing: Chongqing University of Technology(重庆：重庆理工大学)，2010. [10] ZHOU Ming-cheng, YU Ru-qin(周名成，俞汝勤). UV and Visible Spectrophotometric Method(紫外与可见分光光度分析法). Beijing: Chemical Industry Press(北京：化学工业出版社)，1986.
