应用血红蛋白的拉曼光谱进行食管癌诊断的研究

doi:10.3964/j.issn.1000-0593(2013)11-2989-04

摘要
参考文献
相关文章 (15)

全文: PDF (1709 KB)
输出: BibTeX | EndNote (RIS)

摘要：早期诊断对食管癌患者的治疗具有重要意义。通过对21例食管癌患者和20例健康人的血红蛋白溶液进行拉曼光谱的检测和研究，发现食管癌患者与健康人血红蛋白的拉曼光谱有较明显的区别。与健康人相比，食管癌患者血红蛋白中处于低自旋态的铁离子增多，而处于高自旋态的铁离子减少，说明癌变导致食管癌患者血红蛋白中部分处于高自旋态的铁离子转移到了低自旋态，这与癌症患者血样较易溶血的现象相吻合。采用主成分分析(PCA)方法，对所得拉曼光谱建立多元统计模型进行计算，得到的光谱峰值的PCA得分三维散点图可以良好的区分食管癌患者与健康人。利用判别分析方法得出该算法诊断食管癌的灵敏度和特异性分别为90.5%和95%，总判别正确率为92.7%。结果表明，对血红蛋白的拉曼光谱进行检测和分析有可能成为食管癌早期诊断的有效工具之一。

关键词：光谱学;食管癌;拉曼光谱;血红蛋白;早期诊断

Abstract：The Raman spectrums of hemoglobin from 20 normal persons and 21 esophageal cancer patients were measured and analyzed in the present paper, the results show obvious differences between the Raman spectrums of esophageal cancer patients and normal persons. Compared with normal persons, there are more iron ions in low spin state and less in high spin state in the hemoglobin of esophageal cancer patients. It indicates that some iron ions in high spin state turned to low spin state because of cancerization, and this experimental result coincides with the fact that hemolysis is more likely to occur in cancer patients. Principal component analysis(PCA) was employed to get a three-dimensional scatter plot of PC scores for the health and cancer groups, and it can be learned that they are distributed in separate areas. By using the method of discriminate analysis, it was found that the diagnostic algorithm separates the two groups with sensitivity of 90.5% and diagnostic specificity of 95%, and the overall diagnostic accuracy was 92.7%.The results from this exploratory study demonstrate that Raman spectroscopy combined with multivariate analysis would be an effective method for early diagnosis of esophageal cancer.

Key words：Spectroscopy;Esophageal cancer;Raman spectra;Hemoglobin;Early diagnosis

收稿日期: 2013-03-20 修订日期: 2013-06-16

中图分类号:

O657.3

通讯作者: 唐伟跃 E-mail: tangwy@zzu.edu.cn

引用本文:

周雪¹，陈光意²，张建民¹，范春珍¹，梁二军¹，郭嬿²，唐伟跃^1* . 应用血红蛋白的拉曼光谱进行食管癌诊断的研究 [J]. 光谱学与光谱分析, 2013, 33(11): 2989-2992.
ZHOU Xue¹, CHEN Guang-yi², ZHANG Jian-min¹, FAN Chun-zhen¹, LIANG Er-jun¹, GUO Yan², TANG Wei-yue^1* . Research on Early Diagnosis of Esophageal Cancer by Raman Spectroscopy of Human Hemoglobin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(11): 2989-2992.

链接本文:

https://www.gpxygpfx.com/CN/10.3964/j.issn.1000-0593(2013)11-2989-04 或 https://www.gpxygpfx.com/CN/Y2013/V33/I11/2989

[1] Wang G Q. Ann. Thorac. Surg.，2004，77(5)：1740.
[2] LI Xiao-zhou, YANG Tian-yue(李晓舟, 杨天月). The Journal of Light Scattering(光散射学报), 2011, 23(2): 138.
[3] ZHANG Guang-shui, CHEN Chang-qing, QI Jian, et al(张广水, 陈长青, 齐健, 等). Chinese Journal of Lasers(中国激光), 2009, 36(2): 509.
[4] LIN Ju-qiang, HUANG Rui-xiang, LI Yong-zeng, et al(林居强, 黄瑞香, 李永增, 等). Chinese Journal of Lasers(中国激光), 2009, 36(10)：2647.
[5] XIONG Yang, LI Yun-tao, GUO Yan, et al(熊洋, 李云涛, 郭嬿, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(9): 2427.
[6] Nabiev I R，Efremov R G，Chumanov G D. Sov. Phys，Usp.，1988，31：241.
[7] CUI Zi-jian, YAO Yu-lu, WANG Yan, et al(崔子健, 姚雨露, 王艳，等). The Journal of Light Scattering(光散射学报), 2012, 24(1): 40.
[8] G.D.Chumanov G D, Efremov R G, Nabiev I R. Journal of Raman Spectroscopy, 1990, 21: 43.
[9] SHEN Gao-shan, GU Huai-min, YAN Tian-xiu, et al(沈高山, 谷怀民, 闫天秀, 等). Chinese Journal of Lasers(中国激光), 2008, 35(9): 1432.
[10] Tomoko Yamamoto, Graham Palmer. The Journal of Biological Chemistry, 1973, 248(14): 5211.
[11] Hu S, Smith K M, Spiro T G, et al. Am. Chem. Soc., 1996, 118(50): 12638.
[12] WANG Gui-wen, PENG Li-xin, CHEN Ping, et al(王桂文, 彭立新, 陈萍，等). Chinese Journal of Lasers(中国激光), 2009, 36(10): 2651.
[13] ZHUANG Hong, ZHANG Tie-hua, LIN Song-yi, et al(庄红, 张铁华, 林松毅, 等). Food Science(食品科学), 2006, 27(12): 901.
[14] TU Li-yun, ZOU Yong-xin(涂丽云，邹永炘). Journal of Guangxi Agricultural College(广西农学院学报), 1985, 2(2): 49.
[15] DING Xun-jie, SHEN Di, LIN Bao-jue(丁训杰, 沈迪, 林宝爵). Applied Hematology(实用血液病学). Shanghai: Shanghai Medical University Press(上海：上海医科大学出版社), 1992. 119.