Application of FTIR Spectrometry to Monitoring Chemosensitivity of Breast Cancer Cell Line Using 5-FU
HU Hai-jian1,2, ZU Chao1, GUO Lin2,3, HU Qing-nan4, WANG Jian-sheng1*, XU Yi-zhuang2*, WU Jin-guang2
1. First Hospital of Xi’an Jiaotong University, Xi’an 710061, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 3. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China 4. The People’s Hospital of Dengfeng, Dengfeng 452470, China
Abstract：The purpose of the present study was to compare the changes of infrared spectrum of breast cancer cell line MCF-7 treated by 5-fluorouracil with various concentrations and treating time. The results indicate that the relative intensity ratios of I2 920/I1 460 increased in the FTIR spectra of cell line MCF-7 while I1 400/I1 460, I1 080/I1 550 and I1 240/I1 550 decreased significantly within forty-eight hours, however, no regular change was observed during 48～72 hours. The relative intensity ratio of I1 640/I1 550 decreases along with the increase in the concentration of 5-fluorouracil. The changes of infrared spectra are consistent with the biochemical changes in breast cancer cell, and it can be the reference designators when assessing curative effect of breast cancer cell by FTIR spectrometry.
Key words：Breast cancer cell;5-FU;Infrared spectroscopy;Monitor
胡海舰1,2，祖 超1，郭 琳2,3，胡庆楠4，王健生1*，徐怡庄2*，吴瑾光2 . 傅里叶变换红外光谱实时监测乳腺癌细胞化疗反应性的研究 [J]. 光谱学与光谱分析, 2013, 33(11): 2935-2939.
HU Hai-jian1,2, ZU Chao1, GUO Lin2,3, HU Qing-nan4, WANG Jian-sheng1*, XU Yi-zhuang2*, WU Jin-guang2 . Application of FTIR Spectrometry to Monitoring Chemosensitivity of Breast Cancer Cell Line Using 5-FU. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(11): 2935-2939.
[1] Beena Devi1 C R, Tang T S, Corbex M. International Journal of Cancer, 2012, 131(12): 2869. [2] Ozek N S, Tuna S, Erson-Bensan A E, et al. Analyst, 2010, 135(12): 3094. [3] Yang W Y, Xiao X L, Tan J, et al. Vibrational Spectroscopy, 2009, 49(1): 64. [4] Huleihel M, Salman A, Erukhimovitch V, et al. Journal of Biochemcal and Biophysical Methods, 2002, 50(2-3): 111. [5] Sindhuphak R, Issaravanich S, Udomprasertgul V, et al. Gynecologic Oncology, 2003, 90(1): 10. [6] ZHOU Su, XU Zhi, LING Xiao-feng, et al(周 苏, 徐 智, 凌晓锋, 等). Chinese Journal of Oncology(中华肿瘤杂志), 2006, 28(7): 512. [7] Fabian H, Jackson M, Murphy L, et al. Biospectroscopy, 1995, 1(1): 37. [8] Fukuyama Y, Yoshida S, Yanagisawa S, et al. Biospectroscopy, 1999, 5(2): 117. [9] Eckel R, Huo H, Guan H W, et al. Vibrational Spectroscopy, 2001, 27(2): 165.
