Improved Data Processing Method of Fluorescence Correlation Spectroscopy
WANG Shuo1,JIN Lei2*,CHEN Die-yan1
1. Key Laboratory of Atomic and Molecular Nanosciences of Ministry of Education, Department of Physics, Tsinghua University, Beijing 100084, China 2. Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong, China
Abstract：By the unique ability of measuring concentration and diffusion coefficient, fluorescence correlation spectroscopy (FCS) has kept enlarging and deepening its application fields which need measuring physicochemical properties in complex mixtures. However, the classical data processing method in FCS generally induces errors in the fitting parameters, and the intuitionistic information in the chart is little or confused. Aiming at the above problems, we tried a new data processing method with differential treatment to find out whether it has multi-fluorescent-components and estimate the parameters intuitionally in the chart. The differential curves of treated autocorrelation curves have various positions, depths and full widths at half depth of the trough, by which the characteristic diffusion time and the number of the fluorescent components are decided. This improved processing method provides help for the FCS measurement in complex environment of life.
[1] Yoshida N, Kinjo M, Tamusa M. Biochemical and Biophysical Research Communications, 2001, 280: 312. [2] Lenne P F, Colombo D, Giovannini H, et al. Single Moleculars, 2002, 3(4): 194. [3] Sengupta P, Balaji J, Maiti S. Methods, 2002, 27: 374. [4] Mayboroda O A, Van Remoortere A, Tanke H J, et al. Journal of Biotechnology, 2004, 107(2): 185. [5] Berland K M. Journal of Biotechnology, 2004, 108(2): 127. [6] Enderlein J, Gregor I, Patra D, et al. Current Pharmaceutical Biotechnology, 2004, 5: 155. [7] LIU Guang, CHEN Bo, MENG Fan-bo, et al(刘 广，陈 波，孟凡波，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(11): 1379. [8] Dittrich P S, Schwille P. Applied Physics B, 2001, 73: 829. [9] Starchev K, Ricka J, Buffle J. Journal of Colloid and Interface Science, 2001, 233: 50. [10] Elson E L, Magde D. Biopolymers, 1974, 13: 1. [11] Maiti S, Haupts U, Webb W W. Proc. Natl. Acad. Sci. USA, 1997, 94: 11753. [12] Gosch M, Rigler R. Advanced Drug Delivery Reviews, 2005, 57: 169. [13] Ming Zhao, Lei Jin, Bo Chen, et al. Applied Optics, 2003, 42(19): 4031.
