Synthesis of Folate Receptor-Targeted Nanoprobe for Detection of Cancer Cells and Its Spectral Analysis
YAO Cui-ping1*, WANG Jing1*, YANG Yang1, DONG Yan-hua1, XUE Yang1, MEI Jian-sheng1, ZENG Wei-hui2, ZHANG Zhen-xi1*
1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China 2. Department of Dermatology, the Second Affiliated Hospital，Medical School of Xi’an Jiaotong University，Xi’an 710061, China
Abstract：Folate receptor (FR) is particularly upregulated in many epithelial cancer cells membrane and limited distribution is found in normal tissues. In the present work, the folic acid protected gold nanoparticles (FA-GNPs) were synthesized by a simple and quick method, in which chloroauric acid (HAuCl4) was reduced by sodium borohydride (NaBH4) in the presence of FA is used as stabilizer. UV-Visible spectroscopy and transmission electron microscopy (TEM) were used to characterize the shape and size distribution of the produced FA-GNPs. X-ray photoelectron spectroscopy and cell experiment were employed to confirm the immobilization of FA and GNPs. The results showed that FA-GNPs have a good size distribution in the 3~5 nm diameter range. Moreover, it is very stable even in solution with high concentration of salt (up to 3.5% NaCl), and even high speed centrifuges of 25 000 r·min-1 could not cause aggregation. The nanoparticles could be used to detect cancer cells.
姚翠萍1*，王 晶1*，杨 洋1，董艳花1, 薛 阳1，梅建生1，曾维惠2, 张镇西1* . 叶酸受体靶向肿瘤细胞纳米探针的制备及其光谱分析 [J]. 光谱学与光谱分析, 2013, 33(05): 1299-1303.
YAO Cui-ping1*, WANG Jing1*, YANG Yang1, DONG Yan-hua1, XUE Yang1, MEI Jian-sheng1, ZENG Wei-hui2, ZHANG Zhen-xi1*. Synthesis of Folate Receptor-Targeted Nanoprobe for Detection of Cancer Cells and Its Spectral Analysis . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(05): 1299-1303.
