1. State Key Lab of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094,China 2. Out-Patient Department of Beijing Aerospace City, Beijing 100094,China
Abstract:Taking the serum obtained from daily clinical inspection as study object, the Raman spectra were collected with the range of 250 to 2 400 cm-1. Taking the serum cholesterol content determined by traditional clinical biochemical method as contrast, together with combination of PLS, we established a Raman spectroscopy quantitative models for cholesterol content in serum. The experimental results show that Raman spectroscopy displacement could reflect certain chemical group in organic compound. The correction model of cholesterol content in serum was established with the coefficient R=0.909 6, cross-validation calibration standard deviation (RMSECV) of 0.24, standard error of prediction of test set (RMSEP) was 0.69, and coefficient (R) is 0.926 2. The results showed that application of Raman spectroscopy combined with chemometrics in the rapid noninvasive detection of serum cholesterol content is feasible.
Key words:Raman spectroscopy;Partial least squares;Serum;Cholesterol
[1] CHU Xiao-li(褚小立). Chemometric Methods and Molecular Spectroscopy(化学计量学方法与分子光谱分析技术). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2011. 318. [2] YAN Yan-lu, ZHAO Long-lian, HAN Dong-hai, et al(严衍禄,赵龙莲,韩东海,等). Near Infrared Spectroscopy Foundation and Application(近红外光谱分析基础与应用). Beijing: China Light Industry Press(北京: 中国轻工业出版社), 2005. [3] Kelly Virkler, Igor K. Forensic Science International, 2008, 181: 1. [4] Charlotte Eliasson, Neil A Macleod, Linda C Jayes, et al. Journal of Pharmaceutical and Biomedical Analysis, 2008, 47: 221. [5] Ye Qing, Xu Qinfeng, Yu Yongai, et al. Optics Communications, 2009, 282: 3785. [6] Xie Yong, Jiang Yanan, Dor Ben-Amotz. Analytical Biochemistry,2005, 343: 223. [7] Barthus R C, Poppi R J. Vibrational Spectroscopy, 2001, 26(1): 99. [8] Wolfgang Forner, Hassan M Badawi. Journal of Molecular Structure, 2004, 677: 179. [9] Kolomenskii A A, Schuessler H A. Spectrochimica Acta Part A, 2005, 61: 647. [10] Zhang Z L, Yin Y F, Jiang J W, et al. Journal of Molecular Structure, 2009, 920: 297. [11] Moger J, Gribbon P, Sewing A, et al. Biochimica et Biophysica Acta, 2007, 1770: 912. [12] Esam M A Ali, Howell G M Edwards, Michael D. Hargreaves, et al. Analytica Chimica Acta, 2008, 615: 63. [13] Alajtal A I, Edwards H G M, Elbagerma M A, et al. Spectrochimica Acta Part A, 2010, 76: 1. [14] Catinella S, Lipreri M, Castiglioni C, et al. Journal of Molecular Structure, 2006, 788: 126. [15] Debabrata Mandall, Misao Mizuno, Tahei Tahara. Journal of Molecular Structure, 2005, (735-736): 189. [16] LI De-ying, SUN Fang, QIU Jian-ding, et al(李德英,孙 芳,邱建丁,等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(2): 187.