Synchrotron Radiation-Based FTIR Microspectroscopy Study of 6-Hydroxydopamine Induced Parkinson’s Disease Cell Model
ZHU Hong-yan1*, PEI Xiao1, WU Ling-yan1, QI Ze-ming2, WANG Yu-yin2, LIU Bo3, ZHOU Hou-guang4
1. Laboratory of Neuropharmacology and Neurotoxicology, School of Life and Science, Shanghai University, Shanghai 200444,China 2. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, China 3. Department of Physics, Tongji University, Shanghai 200092, China 4. Fudan University Affiliated Huashan Hospital, Shanghai 200040, China
Abstract：SH-SY5Y cell line treated with 6-hydroxydopamine (6-OHDA) is a classical Parkinson’s disease (PD). In the present study, synchrotron-based Fourier transform infrared (FTIR) microspectroscopy was used to analyze the biochemical composition of SH-SY5Y cell line treated with 6-OHDA. The detailed spectral analyses show the significant changes in cellular compositions such as lipids, proteins and nucleic acids in SH-SY5Y cells treated with 6-OHDA compared to control SH-SY5Y cells. As a result, the unsaturation levels of phospholipids decrease in SH-SY5Y cells treated with 6-OHDA compared to control cells, the analysis of protein secondary structure shows the significantly higher ratio of β-sheet in PD cells compared to that of control cells, and the content of nuclear acid is highly decreased compared to that of control cells, suggesting that 6-OHDA induces the serious oxidative damage in SH-SY5Y cells. These findings suggest that SR-FTIR is an effective and precise technical tool to probe the biochemical changes of cells and then evaluate the pathological damage in cells.
[1] Dumas P, Sockalingum G D, Sule-Suso J. Trends in Biotechnology, 2006, 25(1): 40. [2] Miller L M, Dumas P. Biochimica et Biophysica Acta, 2006, 1758: 846. [3] ZHU Hong-yan, WU Ling-yan, PEI Xiao, et al(朱红艳, 吴凌燕, 裴 虓，等). Spectroscopy and Spectroscopy Analysis(光谱学与光谱分析), 2012, 32(1): 113. [4] Prolla T A, Mattson M P. Trends Neurosci, 2001, 24(11 Suppl): S21. [5] Hillion J M, Canals M, Torvinen M, et al. J. Biol. Chem., 2002. 277(20): 18091. [6] Heikkila R E, Sonsalla P K, Duvoisin R C. Neuromethods, 1989. 12: 351. [7] Petibois C, Deleris G. Trends Biotechnology, 2006, 24(10): 455. [8] Petibois C, Deleris G. Cell Biol. Int., 2005, 29(8): 709. [9] Chen C T, Green J T, Orr S K, et al. Prostaglandins Leukot Essent Fatty Acids, 2008. 79(3-5): 85. [10] Darios F, Davletov B. Nature, 2006, 440(7085): 813. [11] Klivenyi P, Beal M F, Ferrante R J, et al. J. Neurochem., 1998, 71(6): 2634. [12] Farooqui A A, Horrocks L A. Cell Mol. Neurobiol., 1998. 18(6): 599. [13] Bandekar J. Biochim. Biophys. Acta, 1992, 1120(2): 123. [14] Krimm S, Bandekar J. Adv. Protein Chem., 1986, 38: 181. [15] Ross C A, Poirier M A. Nat. Med., 2004, 10(Suppl): S10. [16] Welzel A T, Walsh D M. Ir J. Med. Sci., 2011. 180(1): 15. [17] Sahu R K, Mordechai S, Manor E. Biopolymers, 2008, 89(11): 993. [18] Perumal A S, Gopal V B, Tordzro W K, et al. Brain Res. Bull., 1992. 29(5): 699.
