Spectral Analysis of Formation of Trihalomethanes during Reaction of Tryptophan with Disinfectants
HUANG Xin1, WU Xue-fei1, GAO Nai-yun2*, LU Ning3
1. School of Environment and Chemical Engineering, Shanghai University, Shanghai 200444, China 2. State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China 3. Shanghai Engineering Research Center of Urban Water Resources, Shanghai 200082, China
摘要： 使用荧光光谱和紫外光谱分析消毒剂(自由氯，自由溴)和色氨酸的反应及消毒副产物三卤甲烷的产生特性。实验表明：消毒剂反应降低了色氨酸荧光强度和FRI值(fluorescence region integration)，二者线性相关。消毒剂中自由溴比例增加，荧光团FRI降低，UV280值降低，UV254值增加。THMs中溴代产物比例也随自由溴比例单调增加，而THMs总量没有呈现相似的变化规律。反映出和自由氯相比，自由溴取代性更强而氧化性更弱。自由溴单独和色氨酸反应时，FRI与CHBr3产生量线性相关。加入NH3后，消毒剂性质改变使得线性被破坏。
关键词：色氨酸;三卤甲烷;三维荧光光谱;自由溴;消毒
Abstract：UV and fluorescence spectra were applied to analyze the characteristics of reaction of disinfectants (free chlorine, free bromine) with tryptophan, as well as the formation of disinfection byproduct-trihalomethanes (THMs). The results show that disinfecting reaction decrease both the intensity and FRI (fluorescence region integration) of tryptophan. A linear relationship was found between the two indexes. Increasing the ratio of free bromine led to a lowering of FRI, a decrease in UV280 and an increase in UV254. Bromine-substitution ratio of THMs also went up with increasing the ratio of free bromine, whereas the total THMs concentration didn’t follow such mono-increase pattern. Those phenomena are mainly due to a higher substitution but a lower oxidation capacity of free bromine, when compared with free chlorine. A linear relationship between FRI and CHBr3 concentration was found using free bromine alone, which did not exist by dosing ammonia to change the characteristic of disinfectant.
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