Determination of Six Metal Elements in Animal Samples by Flame Atomic Absorption Spectrometry with Wet Digestion
LIU Ming-jia1, 2, ZHOU Zhi-qiang1, 2, ZU Yuan-gang1, 2, ZHANG Yu-hong1, 2*
1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China 2. Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Abstract：The contents of 6 metal elements (Ca, Mg, K, Na, Cu and Fe ) were determined in serum, urine, feces and different tissue and organs (heart, kidney, stomach, liver, spleen, intestine and lung) of Wistar rats and mice by flame atomic absorption spectrometry (FAAS) with wet digestion. The samples were digested by the mixture of HNO3 and HClO4 (4∶1, V/V) at 120 ℃, the correlation coefficient for the standard curves was 0.999 4～0.999 8, the relative standard deviation (RSD) was from 0.33% to 4.52%, and the recovery for the samples was from 97.7% to 104.2%, meeting the demand of elements content determination in biological samples. The assay method for the determination of the 6 metal elements in animal samples established in this study has the advantages of easy operation, high sensitivity, less sample requiring and accurate results, and the method can be widely used in the determination of various metal elements in biological samples.
刘明家1，2，周志强1，2，祖元刚1，2，张玉红1，2* . 湿法消解-火焰原子吸收法测定动物样品中六种金属元素 [J]. 光谱学与光谱分析, 2012, 32(07): 1961-1964.
LIU Ming-jia1, 2, ZHOU Zhi-qiang1, 2, ZU Yuan-gang1, 2, ZHANG Yu-hong1, 2* . Determination of Six Metal Elements in Animal Samples by Flame Atomic Absorption Spectrometry with Wet Digestion . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(07): 1961-1964.
[1] YAN Shi-ming，LI Zeng-xi，XIONG Li-ping(颜世铭，李增禧，熊丽萍). Guangdong Trace Elements Science(广东微量元素科学)，2002，9(9)：1. [2] XIE Yu-da(谢裕达). Journal of Hainan Medical College(海南医学院学报)，2008，14(2)：140. [3] ZOU Li-yi，WU Tie，CUI Liao(邹丽宜，吴 铁，崔 燎). Chinese Pharmacological Bulletin(中国药理学通报)，2002，18(4)：437. [4] YANG Mei，FENG Fang(杨 梅，冯 芳). Progress in Pharmaceutical Sciences(药学进展)，2008，32(2): 56. [5] Wu P, He S P, Luo B, et al. Applied Spectroscopy Review, 2009, 44(5)：411. [6] Chakrapani G, Mahanta P L, Murty D S R, et al. Talanta, 2001, 53(6)：1139. [7] FANG Yan-ling, FANG Yan-min, ZHANG Bo (方艳玲, 方艳敏, 张 波). China Tropical Medicine (中国热带医学 ), 2005，5(9): 1913. [8] Bohrer D, Dessuy MB, Kaizer R, et al. Analytical Biochemistry, 2008, 377(2): 120. [9] Borges D L G, Da Silva A F, Welz B, et al. J. Anal. At. Spectrom., 2006, 21(8): 763.
