Rapid Detection of 6-Benzylaminopurine Residues in Sprout Beans by Surface-Enhanced Raman Spectroscopy
ZHANG Ping1, ZHENG Da-wei1, LIU Jing1, ZHONG Ru-gang1*, LIU Chun-wei2, GUO Xun2, WANG Hong2
1. The College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China 2. OptoTrace Technologies, Inc.，Suzhou 215125, China
Abstract：New method for rapid and quantitative analysis of 6-benzylaminopurine (6-BA) residues in sprout beans was studied by using FAST-SPE and portable surface-enhanced Raman spectroscopy (SERS). With homemade sprout bean samples as blank control, 6-BA solutions extracted from inserted-treatment samples showed typical characteristic Raman peaks at 1 002 cm-1 tested by SERS, and normalized 1 002 cm-1 intensities had a good linear relationship with gradient concentrations of 6-BA insert-standard solutions. The high concentration linear range was 0.5～14 μg·mL-1, and the low one was 0.1～2 μg·mL-1. The quantitative detection limit was down to 0.02 mg·kg-1 that was below the maximum allowable residues (MAL) of 0.2 mg·kg-1 in related National Standard. The recoveries were 82.3%～95.1% and the RSD was below 5%. This method with portable equipment and simple pre-treatment showed good reproducibility, short test time-consuming and easy operating, and thus it would provide efficient and sensitive solutions for large-scale on-site and rapid detections.
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