FTIR Spectra Investigation of Rat Sensitive Tissues Exposure to ELF-EMF
LI Gun1, YAN Ya-jing1, HUANG Yong1, ZHOU Yan2, PANG Xiao-feng1, 3*
1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China 2. Department of Military Preventive Medicine, the Fourth Military Medical University, Xi’an 710032, China 3. International Centre for Materials Physics, Chinese Academy of Sciences, Shenyang 110015, China
Abstract：Nonthermal effects of extremely low frequency electromagnetic fields (ELF-EMF) are related to changes in the microstructure of biological tissues after exposure to electromagnetic radiation. Fourier transform infrared spectroscopy (FTIR) analysis method can effectively study the mechanisms of biological effects of electromagnetic radiation. The present paper studies the characteristics of sensitive tissues of rat after ELF-EMF exposure. FTIR analysis of sensitive tissues of control group and experimental group showed that 50 Hz, 0.75 mT ELF-EMF exposure for 20 days can affect molecular level of the rat testis, blood and brain. Experimental results show that regular changes in absorption peak positions and intensity in the spectra of sensitive tissue may be caused by exposure to ELF-EMF, therefore, Fourier transform infrared spectroscopy is an effective means to study mechanism of biological effects of electromagnetic fields.
Key words：Infrared spectroscopy;Biological effects;Extremely low frequency electromagnetic fields (ELF-EMF)
[1] Wertheimer N, Leeper E. American Journal of Epidemiology, 1979, 109(3): 273. [2] Emanuele C, Salvatore C, Salvatore M, et al. J. Electromagnetic Analysis & Applications, 2011, 3(2): 69. [3] ZHANG An-ying, YUAN Ping, ZHOU Yu-rong, et al(张安英, 袁 平, 周玉荣, 等). Journal of Biomedical Engineering(生物医学工程学杂志), 2009, 26(2): 248. [4] Kim S, Im W. Cell Biochemistry and Biophysics, 2010, 57(1): 1. [5] Bernadette T, Alfonsina C, Elisa P, et al. Bioelectromagnetics, 2006, 27(7): 560. [6] Yoon W K, Hee S K, Jin S L, et al. Bioelectromagnetics, 2009, 30(1): 66. [7] Kajiwara K, Fukushima H, Kishikawa H, et al. Medical Progress Through Technology. 1992, 18(3): 181. [8] Shen Y C, Davies A G, Linfield E H, et al. Journal of Biological Physics，2003, (29): 129. [9] Vallejo D, Torre M, Sanz P, et al. Electromagnetic Biology and Medicine, 2003, 22(2/3): 133. [10] Jelenkovica A, Janaca B, Pesica V, et al. Brain Research Bulletin, 2006, 68(5): 355. [11] PEI Jian, XIE Tao-rong, YAN Zhe, et al(裴 剑, 谢涛嵘, 严 喆, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2011, 31(6): 1537. [12] DENG Zhao-hui, GONG Qian-fen, YU Zheng-ping, et al(邓朝晖, 龚茜芬, 余争平, 等). Chinese Journal of Radiological Health(中国辐射卫生), 2008, 17(2): 136. [13] James J G, William J S, Mullins J M, et al. Biochemical and Biophysical Research Communications, 1991, 174(2): 742. [14] Hashish A H, El-Missiry M A, Abdelkader H I, et al. Ecotoxicology and Environmental Safety, 2008, 71(3): 895. [15] Fabriziomaria G, Annalisa B, Meri S, et al. Science of the Total Environment, 2009, 407(3): 1218. [16] Minder C E, Pfluger D H. American Journal of Epidemiology, 2001, 153(9): 825. [17] YU Hai-tao, WANG Jiang, DENG Bin, et al(于海涛, 王 江, 邓 斌, 等). Acta Biophysica Sinica(生物物理学报), 2010, 26(10): 907. [18] DU Xiao-gang, XU Shan-shan, CHEN Qing, et al(杜晓刚, 徐珊珊, 陈 苘, 等). Journal of Zhejiang University(Medical Sciences)(浙江大学学报·医学版), 2008, 37(1): 9. [19] PAN Qing-hua, WAN Wei-qing, JIA Gui-jun, et al(潘庆华, 万伟庆, 贾桂军, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2010, 31(9): 1717.
