Confocal Raman Microspectroscopy Study on the Distribution of Cellulose and Lignin in Daphne odora Thunb.
ZHANG Zhi-heng1, MA Jian-feng1, XU Feng1, 2*
1. School of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China 2. School of Light and Textile Industry, Qiqihar University, Qiqihar 161006, China
Abstract：Confocal Raman microspectroscopy is well suited to investigating cellulose and lignin distribution in-situ in the native cell walls of woody tissue. In this study, transmission electron microscopy (TEM) was used to determine the ultrastructure of Daphne odora Thunb.. In the TEM images, cell wall of Daphne odora Thunb. is typically divided into three layers: middle lamellar (ML), primary wall (P) and secondary wall (S1, S2 and S3). More detailed information about cellulose and lignin distribution in different cell wall layers was analyzed in situ by confocal Raman microspectroscopy. Raman spectra and images reveal that the distribution of cellulose and lignin in the cell wall layers is not uniform. Lignin concentration in different morphological areas follows the decreasing order: the cell corner (CC)> the middle lamellar (CML)> the secondary wall (S2). In contrast, cellulose distribution shows the opposite pattern-low concentration in CC and CML and high in S2 regions.
Key words：Daphne odora Thunb.;Ultrastructure;Raman microspectroscopy;Cellulose distribution;Lignin distribution
张智衡1，马建锋1，许 凤1, 2* . 结香纤维素与木素分布的显微激光拉曼光谱研究[J]. 光谱学与光谱分析, 2012, 32(04): 1002-1006.
ZHANG Zhi-heng1, MA Jian-feng1, XU Feng1, 2* . Confocal Raman Microspectroscopy Study on the Distribution of Cellulose and Lignin in Daphne odora Thunb. . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(04): 1002-1006.
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