Rapid Diagnostics of Early Phosphorus Deficiency in Mini-Cucumber Plants under Protected Cultivation by Near Infrared Spectroscopy
SHI Ji-yong1, ZOU Xiao-bo1*, ZHAO Jie-wen1, MAO Han-ping2, WANG Kai-liang1, CHEN Zheng-wei1, HUANG Xiao-wei1
1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China 2. Key Laboratory of Modern Agricultural Equipment and Technology, Jiangsu University, Zhenjiang 212013, China
Abstract：The morphological symptom of phosphorus deficiency at early stage is similar to the appearance of leaf aging process in preliminary phase, so that visual diagnostics of phosphorus deficiency in mini-cucumber plants at early stage is practically impossible. Near infrared reflectance spectra contain information about differences in compositions of leaf tissues between phosphorus-deficient plants and healthy plants. In the present paper, near infrared reflectance spectroscopy was used to provide diagnostic information on phosphorus deficiency of mini-cucumber plants grown under non-soil conditions. Near infrared spectra was collected from 90 leaves of mini-cucumber plants. Raw cucumber spectra was preprocessed by SNV and divided into 27 intervals. The top 10 principal components (PCs) were extracted as the input of BP-ANN classifiers by principal component analysis (PCA) while the values of nutrient deficient were used as the output variables of BP-ANN and three layers BP-ANN discrimination model was built. The best experiment results were based on the top 3 principal components of No.7 interval when the spectra was divided into 27 intervals and identification rates of the ANN model are 100% in both training set and the prediction set. The overall results show that NIR spectroscopy combined with BP-ANN can be efficiently utilized for rapid and early diagnostics of phosphorus deficiency in mini-cucumber plants.
Key words：Diagnostics of deficiency;Near infrared spectroscopy;Phosphorus;Mini-cucumber plants;BP-ANN
石吉勇1，邹小波1*，赵杰文1，毛罕平2，王开亮1，陈正伟1，黄晓玮1 . 基于近红外光谱的设施栽培水果黄瓜磷元素亏缺初期快速诊断[J]. 光谱学与光谱分析, 2011, 31(12): 3264-3268.
SHI Ji-yong1, ZOU Xiao-bo1*, ZHAO Jie-wen1, MAO Han-ping2, WANG Kai-liang1, CHEN Zheng-wei1, HUANG Xiao-wei1. Rapid Diagnostics of Early Phosphorus Deficiency in Mini-Cucumber Plants under Protected Cultivation by Near Infrared Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(12): 3264-3268.
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