Abstract:The interaction mechanisms between isoflavones (Genistein, 3’,4’,7-trihydroxyisoflavone and Biochanin A) and different isomers of human serum albumin (HSA) were investigated by fluorescence spectroscopy. Various parameters (quenching rate constants, binding constants and number of binding sites) of isoflavones-human serum albumin complexes were calculated. The results showed that the isoflavones have only one binding site on human serum albumin, located at the Site I, and the binding constants were between 0.17×105 and 1.20×105 L·mol-1. Fluorescence enhancement experiments showed that the fluorescence intensities of the drugs significantly increased after interacting with HSA, indicating that the combination of drug and HSA had occurred. The binding mechanisms between three isoflavones and HSA were discussed.
马 纪,刘 媛,谢孟峡* . 三种异黄酮类药物与不同异构体人血清白蛋白的作用机制研究[J]. 光谱学与光谱分析, 2012, 32(01): 1-6.
MA Ji, LIU Yuan, XIE Meng-xia* . Interaction between Three Isoflavones and Different Isomers of Human Serum Albumin . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(01): 1-6.
[1] Heim K E, Tagliaferro A R, Bobilya D J. J. Nutr. Biochem., 2002, 13: 572. [2] Szkudelska K, Nogowski L. J. Steroid Biochem. Mol. Biol., 2007, 105: 37. [3] Moon Y J, Wang X D, Morris M E. Toxicology in Vitro, 2006, 20: 187. [4] Liu H L, Jiang W B, Xie M X. Recent Pat Anti-canc. Drug Discov., 2010, 5: 152. [5] Dixon R A, Ferreira D. Phytochemistry, 2002, 60: 205. [6] Paradkar P N, Blum P S, Berhow M A, et al. Cancer Lett., 2004, 215: 21. [7] Duan W, Kuo I C, Selvarajan S, et al. Am. J. Respir. Crit. Care Med., 2003, 167: 185. [8] Verdrengh M, Jonsson I M, Holmdahl R, et al. Inflamm. Res., 2003, 52: 341. [9] Ma J, Yin Y M, Liu H L, et al. Curr. Org. Chem., 2011, 15(15): 2627. [10] Qin C, Xie M X, Liu Y. Biomacromolecules, 2007, 8: 2182. [11] Pastukhov A V, Levchenko L A, Sadkov A P. J. Mol. Struct., 2007, 842: 60. [12] Xie M X, Long M, Liu Y, et al. Biochim. Biophys. Acta-Gen. Subj., 2006, 1760: 1184. [13] XIE Meng-xia, XU Xiao-yun, WANG Ying-dian, et al(谢孟峡, 徐晓芸, 王英典, 等). Acta Chimica Sinica(化学学报), 2005, 63(22): 2055. [14] Guharay J, Sengupta B, Sengupta P K. Proteins, 2001, 43: 75. [15] Sytnik A, Litvinyuk I. Proc. National Academy of Sciences of USA, 1996, 93: 12959.