Molecular Hyperspectral Imaging (MHSI) System and Application in Biochemical Medicine
LIU Hong-ying1, LI Qing-li1*, WANG Yi-ting2, LIU Jin-gao1, XUE Yong-qi3
1. Key Laboratory of Polar Materials and Devices, East China Normal University, Shanghai 200241, China 2. Institute for Advanced Interdisciplinary Research (IAIR), East China Normal University, Shanghai 200062, China 3. Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China
Abstract：A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1 000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0.3 μm. This paper has also presented that spectral curves extracted from the corrected hyperspectral data of the sample, which have been preprocessed by the gray correction coefficient, can more truly represent biochemical characteristic of the sample. The system can supply not only single band images in the visible range, but also spectrum curve of random pixel of sample image. This system can be widely used in various fields of biomedicine, clinical medicine, material science and microelectronics.
刘洪英1，李庆利1*，王依婷2，刘锦高1，薛永祺3 . 分子超光谱成像系统及其生物医学应用[J]. 光谱学与光谱分析, 2011, 31(10): 2593-2597.
LIU Hong-ying1, LI Qing-li1*, WANG Yi-ting2, LIU Jin-gao1, XUE Yong-qi3 . Molecular Hyperspectral Imaging (MHSI) System and Application in Biochemical Medicine. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(10): 2593-2597.
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