Study on of Deferrization Mechanism of Apoferritin and the Effect of Spectra Variation with Holoferritin
LIU Wen-ying1,2，ZHANG Jian1, 3*，TONG Jun-mao1，ZHAO Guang-hua3*
1. College of Food, Shihezi University, Shihezi 832003, China 2. Chinese National Egg Engineering Research Center, Beijing 102115，China 3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Abstract：The deferrization mechanism of apoferritin was established, and the spectra variation of apoferritin was compared with that of holoferritin. Sodium hyposulfite is a strong reducing agent, therefore, was applied to deoxidize holoferritin to release iron ion，and connection of iron of buffer was measured by the 2,2-dipyridyl. Apoferritin was detected by ICP-MS. Holoferritin was found to have no absorption compared with apoferritin by UV analysis, and have no fluorescence emission spectra in contrast with apoferritin by fluorescence analysis.
刘文营1,2，张 建1, 3*，童军茂1，赵广华3* . 铁蛋白脱铁机制的建立及脱铁前后光谱变化的研究[J]. 光谱学与光谱分析, 2011, 31(09): 2552-2554.
LIU Wen-ying1,2，ZHANG Jian1, 3*，TONG Jun-mao1，ZHAO Guang-hua3* . Study on of Deferrization Mechanism of Apoferritin and the Effect of Spectra Variation with Holoferritin . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(09): 2552-2554.
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