Study on Measurement of Cholesterol in Serum by Near-Infrared Spectroscopy and Applicability of Models
YANG Hao-min1, 2, LU Qi-peng1*
1. State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033，China 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：To study rapid analysis of cholesterol concentration in human serum with no reagent, near-infrared spectroscopy was used. Applicability of analytical models was studied. Spectra of serum were measured by a FT-NIR spectrometer with 1, 2 and 6.5 mm optical path length respectively. Partial least-square (PLS) models were calibrated for cholesterol in combination, first overtone and second overtone spectral regions. Root mean square error of prediction (RMSEP) of these models is 0.15, 0.16 and 0.29 mmol·L-1, and mean percent error of prediction (MPEP) is 2.9%, 3.1% and 4.8%, respectively. To validate applicability of these models, other two groups of serum spectra were measured in one month after the models were calibrated. These two sample groups were calculated using calibrated models. Prediction result of 1mm model is the best. The result of this experiment indicates that it’s possible to calibrate precise models for cholesterol. If model is based on thinner samples, applicability is better.
杨皓旻1, 2，卢启鹏1*. 血清中胆固醇的近红外光谱测量及模型适用性研究[J]. 光谱学与光谱分析, 2011, 31(02): 375-378.
YANG Hao-min1, 2, LU Qi-peng1* . Study on Measurement of Cholesterol in Serum by Near-Infrared Spectroscopy and Applicability of Models. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(02): 375-378.
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