The Production of Hydroxyl Radical in HRP-NADH-H2O2 /O2 Systems and Its Application in Chlorobenzene Removal
LI Hai-tao1,2, LI Yu-ping2, CAO Hong-bin2*, LI Xin-gang1, XUE Zhan-qiang3
1. School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China 2. Key Laboratory of Green Process & Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 3. Chemical Plant, Angang Steel Co. Ltd., Anshan 114021, China
Abstract：The mechanism of radical generation in HRP-NADH-O2/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements, and the novel enzyme-coenzymatic systems were performed to degrade chlorobenzene as a non-phenolic persistent organic pollutants. The UV results showed that compound Ⅲ was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH, which would generate hydroxyl radical. The ESR results demonstrated the production of ·OH and O-2· in enzyme-coenzymatic system in the presence of O2 or H2O2 with DMPO and POBN as spin-trappers, respectively. In HRP-NADH-H2O2 system, compound Ⅲ was the main state of HRP in the initial 10 min, and then converted to HRP with generating hydroxyl radical; and after the addition of oxygen, the production of hydroxyl radical was promoted rapidly, as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn-Cu) decreased the production of hydroxyl radical significantly, resulting from that SOD eli minated O2 reduction to O-2· by NADH and then inhibited ·OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal. The removal of chlorobenzene with HRP-NADH-H2O2 and HRP-NADH-H2O2-O2 systems reached 24.6% and 48.2%, respectively, which was much higher than that with traditional enzymatic system (1.42%), showing a promising prospect in proposal of other non-phenol POPs in wastewater.
李海涛1,2，李玉平2，曹宏斌2*，李鑫钢1，薛占强3 . 过氧化物酶-辅酶NADH催化O2/H2O2产生羟基自由基研究及其氯苯处理初探 [J]. 光谱学与光谱分析, 2010, 30(11): 3119-3123.
LI Hai-tao1,2, LI Yu-ping2, CAO Hong-bin2*, LI Xin-gang1, XUE Zhan-qiang3 . The Production of Hydroxyl Radical in HRP-NADH-H2O2 /O2 Systems and Its Application in Chlorobenzene Removal . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(11): 3119-3123.
