Speciation Analysis of Trace Elements Cu,Fe and Zn in Serum by Flame Atomic Absorption Spectrophotometry
HU Jun1,CHANG Yao-ming1*,GAO Shuang-bin2,HAI Chun-xu3,LI Jin-sheng1,XIE Xiao-ping1
1. Department of Aerospace Hygiene,School of Aerospace Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China 2. Department of Nutriology,School of Preclinical Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China 3. Department of Toxicology,School of Preclinical Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China
Abstract：Since biological functions of the elements are generally different,depending on their chemical forms,chemical speciation analysis is really important in metallomics research. Thus,multielement analysis and chemical speciation of the elements in serum were carried out in the present work. A hyphenated technique was developed for high-throughput speciation analysis of the copper,iron and zinc in serum by molecular biology technology and flame atomic absorption spectrophotometry (AAS). Here,Cu,Fe and Zn in serum were classifyied as the forms of combination and non-combination. The serum protein was precipitated by 60% concentration of ethanol under hypothermy. The forms of combination of Cu,Fe and Zn in serum which combined with proteins were in precipitations,and the forms of non-combination of Cu,Fe and Zn in serum,which were free ions,were in supernatant. The total amount of Cu,Fe and Zn in serum and the amount of the forms of non-combination of Cu,Fe and Zn were analyzed by AAS. The amount of the forms of combination of Cu,Fe and Zn was obtained by calculation. The detection limit of Cu in serum by the method is around and 9.84×10-3 μg·mL-1. For Fe and Zn,the detection limit is about 2.76×10-2 μg·mL-1 and 1.06×10-3 μg·mL-1,respectively. The percentage recovery of trace elements Cu,Fe and Zn by the proposed procedure is in the range 95.0%-101.0%，95.0％-102.0％ and 95.0％-103.0％,respectively. The relative standard deviation(RSD) of trace elements Cu,Fe and Zn in the serum is in the range 1.88％-2.26％，0.56％-1.59％ and 0.34％-1.36％,respectively. Speciation of trace elements Cu,Fe and Zn in the serum of SD rat were analyzed by the method.
[1] CONG Tao,ZHAO Lin(丛 涛，赵 霖). Studies of Trace Elements and Health(微量元素与健康研究),2006,23(6): 59. [2] KAI Xiao-ming(开小明). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(11): 1412. [3] CHEN Jing-yuan(陈景元). Trace Elements and Health(微量元素与健康). Xi’an: Fourth Military Medical University Press(西安: 第四军医大学出版社),2003. 20. [4] ZHANG Wei,ZHANG Zhuo-yong,SHI Yan-zhi,et al(张 薇，张卓勇，施燕支,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(5): 963. [5] WANG Jia-zheng,FAN Ming(汪家政，范 明). Protein Technical Manual(蛋白质技术手册). Beijing: Science Press(北京：科学出版社)，2000. 68. [6] Szpunar J. Analyst,2005,130(4): 442.
