GFAAS测定氧化砷处理移植性食管癌大鼠后各组织的砷浓度

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摘要：用石墨炉原子吸收法测定了大鼠各组织中的砷浓度。在大鼠移植性肿瘤组织内一次性注射10 μg砷后，观察不同时间大鼠各脏器砷的分布。组织通过80℃水浴用硫酸-硝酸-高氯酸混酸体系封闭消解2 h后，滴加30%双氧水，得到定容的消化液。用0.1% Triton X-100-0.2% AgNO₃溶液作消化液的基体改进剂，D₂灯校正背景，标准加入法校准曲线测定。此法相对标准偏差为3.2%～8.7%之间。检出限为1.57 μg·L^-1。回收率为81.7%～105%。分析结果表明，注射到肿瘤组织内的砷2 h内扩散到其他组织达最大值，而后随机体代谢活动的结果，砷浓度随时间延长又渐渐减少。

关键词：砷;大鼠组织;GFAAS

Abstract：As₂O₃ was injected into tumour tissue of esophagoscope-transplant mouse. The concentration of As diffusing into other tissues was investigated. A method was proposed for the determination of As in tissue samples of mouse by graphite furnace atomic absorption spectrometry. After wet digestion (water-bath at 80℃) with 2∶1∶1(φ) of HNO₃-H₂SO₄-HClO₄, the digested tissue samples of mouse were diluted with 0.2% Triton X-100-0.4% AgNO₃. The matrix-matching calibration curve of non-interference was established with standard addition method. The relative standard deviation was 3.2％-8.7%. The limit of detection was 1.57 μg·L^-1. The recoveries were 81.７％-105％ Arsenic concentrations in mouse liver, kidney, brain, left-chest, and right-chest were determined after injection of arsenic at different times.

Key words：Arsenic;Tissue;GFAAS

收稿日期: 2003-01-21 修订日期: 2003-06-26

中图分类号:

O657.31

通讯作者: 张源

引用本文:

张源，罗文鸿，李慧，沈忠英. GFAAS测定氧化砷处理移植性食管癌大鼠后各组织的砷浓度 [J]. 光谱学与光谱分析, 2004, 24(04): 487-490.
ZHANG Yuan，LUO Wen-hong，LI Hui，SHEN Zhong-ying . Determination of Arsenic in As₂O₃-Treated Esophagoscope-Transplant Mouse Tissue by Graphite Furnace Atomic Absorption Spectrometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(04): 487-490.

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