An Optical Mapping System Based on Spectral Shift of Voltage-Sensitive Dyes
WANG Jing1,ZHANG Zhen-xi1*,XU Zheng-hong1,2,JIN Yan-shu1,JI Xiao-lu1,JIN Yin-bin1,2
1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education,and Institute of Biomedical Analytical Technology and Instrumentation,School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,China 2. College of Electrical Engineering,Xi’an Jiaotong Uiversity,Xi’an 710049,China
Abstract：Recently,non-invasive optical methods to monitor transmembrane electrical potential using voltage sensitive dyes have been applied widely in the studies of normal and pathological heart rhythms and defibrillation. In the present paper,the authors measured the excitation and the emission spectra of the voltage-sensitive dyes di-4-ANEPPS bound to phospholipid bilayer membranes. And according to the spectral shift of di-4-ANEPPS,the authors presented an optical mapping system combining a DALSA CCD camera and a LED light source. Using this optical mapping system,the authors could record the action potential duration of the heart cells with high spatial and temporal resolutions. It can be a powerful tool in the study of cardiac arrhythmia mechanisms.
Key words：Optical mapping;Voltage sensitive dyes;Di-4-ANEPPS;Fluorescence spectral shift;CCD camera
王晶1,张镇西1*,徐正红1,2,金妍姝1,季晓璐1,金印彬1,2. 基于ANEP染料荧光光谱迁移的单波长心脏光学标测系统[J]. 光谱学与光谱分析, 2008, 28(03): 617-620.
WANG Jing1,ZHANG Zhen-xi1*,XU Zheng-hong1,2,JIN Yan-shu1,JI Xiao-lu1,JIN Yin-bin1,2. An Optical Mapping System Based on Spectral Shift of Voltage-Sensitive Dyes. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(03): 617-620.
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