Mineral Elements Analysis of Momordica Charantiap Seeds by ICP-AES and Fatty Acid Profile Identification of Seed Oil by GC-MS
LIU Xiao-ru1, 2, DENG Ze-yuan1, 2*, FAN Ya-wei1, LI Jing1, LIU Zhi-han1
1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China 2. Institute of Advanced Study, Nanchang University, Nanchang 330047, China
Abstract：In the present study, a special kind of Momordica charantia seeds produced in Hai Nan was selected and analyzed. Firstly, inductively coupled plasma-atomic emission spectrophotometry (ICP-AES) was used to determine the mineral elements. It was clear that the contents of K, Mg and P are the highest in the seeds; Cr and Zn takes up to 5.65% and 45.45% high, especially, which are rare in plant foods. These minerals, especially Cr and Zn might have a complex effect on those proteins or polysaccharides and form a stronger anticipation of hyperlipidemia, hyperglycemia and cholesterol. Secondly, seed oil was extracted by supercritical CO2 extraction with a yield ratio of 36.89%, and the fatty acids were treated by methylation in alkaline process and purified by thin-layer chromatography, then analyzed by gas chromatography-mass spectrometer (GC-MS) identification. The saturated fatty acids (SFA) take up 36.71%, and mainly are stearic acid; monounsaturated fatty acid (MUFA) is only 3.33% which is dominantly linoleic acid (LA); Polyunsaturated fatty acid (PUFA) accounted for 59.96%, and the α-eleostearic acid takes up 54.26% as the main fatty acids in all. The plentiful α-eleostearic acid leads to strong effects of inhibiting tumor cell proliferation, lowering blood fat, anti-cancer, anti-inflammatory and preventing cardiovascular diseases, and so on. Knowing clearly the mineral elements distribution and identifying the composition of fatty acid, especially the main fatty acids in the oil, are both of great guiding importance to further exploit the clinical and edible value in Momordica charantiap seeds.
刘小如1, 2，邓泽元1, 2*，范亚苇1，李 静1，刘芷含1 . ICP-AES测定苦瓜籽矿质元素及GC-MS鉴定其油脂脂肪酸 [J]. 光谱学与光谱分析, 2010, 30(08): 2265-2268.
LIU Xiao-ru1, 2, DENG Ze-yuan1, 2*, FAN Ya-wei1, LI Jing1, LIU Zhi-han1 . Mineral Elements Analysis of Momordica Charantiap Seeds by ICP-AES and Fatty Acid Profile Identification of Seed Oil by GC-MS . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(08): 2265-2268.
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