Spectroscopic Study of Diazepam Molecularly Imprinted Polymers and Initiative Application to Conductimetric Sensor Based on Molecularly Imprinted Films
LIU Xiao-fang, LI Feng, YAO Bing, WANG Li, LIU Guo-yan, CHAI Chun-yan*
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
摘要： 制备地西泮的分子印迹聚合物，应用紫外光谱(ultraviolet spectra, UV spectra)和红外光谱(infrared spectra, IR spectra)分析该聚合物的识别特性及模板分子和功能单体的结合作用。在丝网印刷电极上原位制备地西泮的分子印迹膜，并在此基础上建立了检测地西泮的电导型传感方法。实验结果表明模板分子与功能单体可形成1∶2型氢键配合物，印迹聚合物中存在可与模板分子通过协同氢键相互作用的官能团，该聚合物对地西泮有高度特异的识别力，电导型传感方法对地西泮的检测限达0.008 mg·L-1，线性范围为0.039～0.62 mg·L-1，可实现现场快速检测。
关键词：地西泮;分子印迹聚合物;光谱分析;电导型传感器
Abstract：The molecularly imprinted polymers were synthesized using diazepam as the template and molecularly imprinted films (MIF) prepared on screen printed electrodes (SPE). The binding mechanism and recognition characteristics of the molecularly imprinted polymers were studied by ultraviolet (UV) spectra and infrared (IR) spectra. In addition, a conductimetric sensor for diazepam was established preliminarily based on diazepam MIF modified SPE. The results of UV spectra indicated that template molecules and functional monomers had formed 1∶2 hydrogen bonding complexes; the results of IR spectra showed that there were some functional groups in the molecularly imprintedpolymers which could interact with the template molecules. The molecularly imprinted polymers manifested highly recognition to diazepam. The response of the conductimetric sensor to the concentration of diazepam displayed a linear correlation over a range of 0.04 to 0.62 mg·L-1 with a detection limit of 0.008 mg·L-1. The sensor is suitable for on-the-spot detection of diazepam.
刘晓芳，李 锋，姚 冰，王 莉，刘国艳，柴春彦* . 地西泮分子印迹聚合物的光谱学特性及其在传感测试中的应用 [J]. 光谱学与光谱分析, 2010, 30(08): 2228-2231.
LIU Xiao-fang, LI Feng, YAO Bing, WANG Li, LIU Guo-yan, CHAI Chun-yan* . Spectroscopic Study of Diazepam Molecularly Imprinted Polymers and Initiative Application to Conductimetric Sensor Based on Molecularly Imprinted Films . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(08): 2228-2231.
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