HAN Hong-wen1, YAN Xun-ling1*, LI Shu-feng1, BAN Ge1, LI Ke1, ZHANG Xia1,YANG Ying2, BING Yan-xia2
1. School of Physical Science and Information Technology, Liaocheng University, Liaocheng 252059, China 2. Liaocheng People’s Hospital, Liaocheng 252000, China
Abstract：The SERS spectra were measured from normal and the diabetic serum. In the diabetic serum, the band of amide II shifted to 1 585 cm-1 and the relative intensity increased 14%, while the relative intensity of 593 cm-1 which belongs to amide Ⅵ reduced 33%. For the protein side chain, the band at 1 368 cm-1 assigned to the “buried” tryptophan shifted to 1 365 cm-1 of the “exposed” and the relative intensity reduced 59%. The relative intensity of 635 cm-1 assigned to the gauche conformation of the C—S decrased 15% and the band at 725 cm-1 increased 58%. These indicate that the structure of the protein changed in the diabetic serum. The relative intensity at 1 449 cm-1 assigned to the lipids characteristic increased 58%. The relative intensity of the glucide characteristic at 1 331, 1 099 and 740 cm-1 increased 35%, 100% and 62%, respectively. So it is indicated that the content of lipids, glucide and protein increased in diabetic. These results may offer a powerful experimental basis for diabetes diagnosis and biochemistry mechanism study.
韩洪文1,闫循领1*,李书锋1,班戈1,李珂1,张霞1,杨颖2,邴艳霞2 . 糖尿病人血清的表面增强拉曼光谱[J]. 光谱学与光谱分析, 2009, 29(02): 399-401.
HAN Hong-wen1, YAN Xun-ling1*, LI Shu-feng1, BAN Ge1, LI Ke1, ZHANG Xia1,YANG Ying2, BING Yan-xia2 . SERS Spectra of Serum from Diabetic. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 399-401.
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