Study on Spectral Properties of the Associated Particle of Berberine-PHLO and Its Analytical Application
KANG Cai-yan1, JIANG Zhi-liang1*, MO Wei-ming2
1. Department of Resource and Environmental Science, Guangxi Normal Universitｙ，Gｕilin 541004，China 2. Department of Chemistry, Guangxi Normal University, Guilin 541004, China
Abstract：In neutral medium, berberine forms a red-violet association microparticle with PHLO which has an maxmimum absorption at 560 nm, Beer’s law is obeyed for berberine in the concentration range of 6.65×10-7-7.71×10-5mol·L-1. Spectral properties of the berberine-PHLO system were studied. In neutral medium, there are a maximum absorption peak at 520 nm and a fluorescence peak at 560 nm for PHLO. Berberine and PHLO combine into a hydrophobic berberine-PHLO association molecule by static gravitation. With stronger Van der Waals and hydrophobic forces among berberine-PHLO molecules, it forms association particle in red-violet which exhibits three resonance scattering peaks at 385, 470 and 586 nm，respectively. The fluorescence quenching at 560 nm is because the PHLO molecule in the body of the particle may not be excited by incident photons, so it does not emit fluorescence.
Key words：Berberine-PHLO association microparticle;Resonance scattering;Berberine;Spectrophotometry
康彩艳1，蒋治良1*，莫蔚明2 . 荧光桃红-小檗碱缔合微粒体系的光谱特性研究及分析应用 [J]. 光谱学与光谱分析, 2004, 24(09): 1089-1092.
KANG Cai-yan1, JIANG Zhi-liang1*, MO Wei-ming2 . Study on Spectral Properties of the Associated Particle of Berberine-PHLO and Its Analytical Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1089-1092.
[1] CHI Jia-ping et al(迟家平等). Traditional Chinese Medicines Research(中成药研究)，1991，13(11)：17. [2] GUO Hong-bing, ZHONG Rong(郭宏炳, 钟 容). Journal of Guangdong Medical College(广东医学院学报)，1998，16(3)：289. [3] LIN Si-lan et al(林似兰等). Acta Pharmaceutic Sinica(药学学报)，1989，24(1)：48. [4] KAN Yu-ming(阚毓铭). The Analysis of Preparation of Traditional Chinese Medicines (first edition)(中药制剂分析·第一版). Nanjing: Nanjing University Press(南京：南京大学出版社), 1992. 258. [5] Liu S P, Liu Z F. Spectrochim. Acta, 1995, 51A: 1497. [6] Liu S P, Luo H Q, Liu Z F. Anal. Chem., 2001, 73: 3907. [7] Jiang Zhiliang, Liu Shaopu, Chen Sa. Spectrochemical Acta, 2002, 58: 2759. [8] LIANG Sheng-wang, LIU Wei(梁生旺，刘 伟). The Quantitative Analysis of Preparation of Traditional Chinese Medicines(中药制剂定量分析). Beijing: China Traditional Chinese Medical Press(北京：中国中医药出版社)，1997. 143. [9] ZOU Jie-ming, WANG Li-sheng, JIANG Zhi-liang，YIN Wen-qing et al(邹节明, 王力生, 蒋治良，尹文清等). Journal of Guangxi Normal University(广西师范大学学报)，2002，20(1)：110. [10] JIANG Zhi-liang, FENG Zhong-wei, LI Ting-sheng et al(蒋治良, 冯忠伟, 李廷盛等). Science in China(Series B)(中国科学·B辑)，2001，31(2)：185. [11] JIANG Zhi-liang, ZHONG Fu-xin, LI Ting-sheng(蒋治良, 钟福新, 李廷盛). Acta Chimica Sinica(化学学报)，2001，59：438. [12] JIANG Zhi-liang, LIU Shao-pu, LIU Qing-ye(蒋治良, 刘绍璞, 刘庆业). Chin. J. Appl. Chem.(应用化学)，2002，19(1)：22. [13] LIANG Ai-hui, ZOU Kie-ming, JIANG Zhi-liang, WANG Li-sheng(梁爱惠, 邹节明, 蒋治良, 王力生). Chin. J. Appl. Chem.(应用化学)，2002，19：768.
