1. 北京大学化学与分子工程学院，北京分子科学国家实验室，北京 100871 2. 中国地质大学材料科学与工程学院，北京 100083 3. University of Saskatchewan，Canada S7N5C9 4. 杭州师范大学有机硅化学与材料重点实验室，浙江 杭州 310015 5. 瑞利分析仪器公司，北京 100016
New Progress in Noninvasive Method of Blood Glucose Measurement Using FT-Mid-IR Spectroscopy
WANG Man-man1, 2, BAI Qian1, 3, PAN Qing-hua1, 4, ZHANG Yuan-fu1, WENG Shi-fu1, ZHOU Feng-shan2, GONG Rong-ye5, WU Hui-zhong5, XU Yi-zhuang1*, WU Jin-guang1
1. College of Chemistry and Molecular Engineering, Beijing National Laboratory for Molecular Sciences, Peking University, Beijing 100871, China 2. School of Material Science and Technology, China University of Geosciences, Beijing 100083, China 3. University of Saskatchewan, Saskatoon S7N5C9, Canada 4. Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou 310015, China 5. Beijing Rayleigh Analytical Instrument Corp., Beijing 100016, China
Abstract：The blood glucose concentrations of volunteers from diabetes patients and healthy adults (all patients and volunteers who joined this experiment gave their consents) were measured by using a modified WQF-200 FTIR spectrometer with a newly designed ATR accessory from the Beijing Rayleigh Analytical Instrument Corp. The determination basis for this technique from the physiological point of view is also discussed based on the experimental results, which indicated that the glucose measured by the FT-Mid-IR-ATR instrument is from the secretions on the skin and glucose components within the body. The secreted glucose components will increase with the time increasing. The authors’ previous study[3-8] demonstrated 1 120 cm-1 band as an index to characterize the blood glucose. During the experiments, the authors used the band of 1 455 cm-1 as internal standard because of its stability, and because the relative intensity of I1 120/I1 455 band possesses the higher sensitivity. Meanwhile, from the spectra, the relative intensity of I1 120/I1 455 band of the glucose in both sources exhibits a linear relationship with blood glucose concentration within the body. The dried blood has the similar spectra as fingers’. The fingers’ spectra will exhibit higher sensitivity if the time of measurement is longer after washing hand, and the results showed that when measured 10 minutes after washing hand, the sensitivity will be higher than that when measured 4 minutes after washing hand. All the results can be used in promoting a convenient, rapid and noninvasive way to monitor the continuous variation of blood glucose concentration of diabetes patients in real time.
[1] LIU Juan，WANG Shang-qi，JIAN Shui-sheng, et al(刘 娟，王尚奇，简水生). Acta Laser Biology Sinica(激光生物学报), 2005，14(5): 393. [2] LI Qing-bo，XU Ke-xin，WANG Yan(李庆波，徐可欣，汪 曣). Journal of Tianjin University(天津大学学报)，2003，36(2): 139. [3] XU Yi-zhuang, ZHANG Yuan-fu, YANG Li-min, et al(徐怡庄，张元福，杨丽敏，等) . Chem. J. Chinese Universities(高等学校化学学报), 2004, 25(2): 348. [4] PAN Qing-hua，WANG Bing-bing，LAI Guo-qiao, et al(潘庆华，王冰冰，来国桥，等). Chem. J. Chinese Universities(高等学校化学学报), 2007, 28(5): 843. [5] ZHAO Ying，ZHANG Yuan-fu，WU Jin-guang, et al(赵 莹，张元福，吴瑾光，等). Chem. J. Chinese Universities(高等学校化学学报), 2005, 26 (6): 1052. [6] SUN Ying, YANG Zhan-lan, WU Jin-guang, et al(孙 颖，杨展澜，吴瑾光，等). Progress in Natural Sciences(自然科学进展), 2002, 12: 806. [7] SUN Ying, FU Xian-bo, WU Jin-guang, et al(孙 颖，傅贤波，吴瑾光，等). Chinese J. Mini Invasive Surgeon(中国微创外科杂志), 2001, 1: 159.
